Antibacterial activity of the lipopetides produced by Bacillus amyloliquefaciens M1 against multidrug-resistant Vibrio spp. isolated from diseased marine animals
Antibacterial activity of the lipopetides produced by Bacillus amyloliquefaciens M1 against multidrug-resistant Vibrio spp. isolated from diseased marine animals
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DOI：
10.1007/s00253-013-5291-1
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发表时间：
2013
影响因子：
5
通讯作者：
Hong-Mei Xu;Y. Rong;Ming-xin Zhao;B. Song;Z. Chi
中科院分区：
文献类型：
--
作者：
Hong-Mei Xu;Y. Rong;Ming-xin Zhao;B. Song;Z. Chi
In this work, the antibacterial activity of the lipopeptides produced byBacillus amyloliquefaciensM1 was examined against multidrug-resistantVibriospp. andShewanella aquimarinaisolated from diseased marine animals. A new and cheap medium which contained 1.0 % soybean powder, 1.5 % wheat flour, pH 7.0 was developed. A crude surfactant concentration of 0.28 mg/ml was obtained after 18 h of 10-l fermentation and diameter of the clear zone on the plate seeded withVibrio anguillarumwas 34 mm. A preliminary characterization suggested that the lipopeptide N3 produced byB. amyloliquefaciensM1 was the main product and contained the surfactin isoforms with amino acids (GLLVDLL) and hydroxy fatty acids (of 12–15 carbons in length). The evaluation of the antibacterial activity of the lipopeptide N3 was carried out againstS. aquimarinaand nine species ofVibriospp.. It was found that all theVibriospp. andS. aquimarinashowed resistance to several different antibiotics, suggesting that they were the multidrug resistance. It was also indicated that all theVibriospp. strains andS. aquimarinawere sensitive to the surfactin N3, in particularV. anguillarum. The results demonstrated that the lipopeptides produced byB. amyloliquefaciensM1 had a broad spectrum of action, including antibacterial activity against the pathogenicVibriospp. with multidrug-resistant profiles. After the treatment with the lipopeptide N3, the cell membrane ofV. anguillarumwas damaged, and the whole cells of the bacterium were disrupted.
