Development of Electrochemical 6-Well Plates and Its Stability as an Immunosensor
Development of Electrochemical 6-Well Plates and Its Stability as an Immunosensor
复制标题
电化学 6 孔板的开发及其作为免疫传感器的稳定性
DOI：
10.1149/1945-7111/ac519e
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发表时间：
2022
影响因子：
3.9
通讯作者：
Zhou, H. Susan
中科院分区：
文献类型：
--
作者：
Cui, Feiyun;Zhou, Zhiru;Qu, Bin;Zhou, Qin;Zhou, H. Susan
开发低成本和多路复用的电化学（EC）生物检测装置势在必行。在此，提出了一种基于聚合物的EC器件，命名为EC 6孔板，并使用非光刻方法制备。聚对苯二甲酸乙二醇酯（PETG）被用作基板和激光切割聚酯（PET）膜被用作掩模图案化的电极。工作电极直径为900 μm，每步修饰仅需1 μl试剂。将带有威尔斯孔的丙烯酸模具（60 μl）粘合到PETG基材上。讨论了参比电极（RE）的微型化。制备了固态Ag/AgCl RE基三电极体系、Au三电极体系（3E）和Au双电极体系（2 E），并将其用于检测毒素B的免疫传感器。采用微分脉冲伏安法（DPV）和电化学阻抗谱（EIS）对传感器的稳定性进行了测试。固态Ag/AgCl RE基系统显示开路电位（OCP）的标准偏差为4.6 mV。3E系统和2 E系统的OCP标准差分别为0.0026 mV和0.32 mV。结果表明，采用3E系统的EC 6孔板是开发EC免疫传感器的理想材料。
Developing low-cost and multiplexing electrochemical (EC) devices for bioassay is imperative. Herein, a polymer-based EC device, named EC 6-well plate, was proposed and fabricated using a non-photolithography method. Polyethylene terephthalate glycol (PETG) was used as a substrate and laser-cut polyester (PET) film was used as a mask for patterning the electrodes. The diameter of the working electrode (WE) was 900 μm, and each WE-modifying step only requires 1 μl of reagent. Acrylic mold with wells (60 μl) was bonded to the PETG substrate. Miniaturization of reference electrodes (RE) was discussed. The solid-state Ag/AgCl RE-based three-electrode system, the Au three-electrode system (3E), and Au two-electrode system (2E) were prepared and employed to develop an immunosensor for toxin B detection. Differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) were applied to test the stability of the EC immunosensor. The solid-state Ag/AgCl RE-based system showed a standard deviation of open circuit potential (OCP) of 4.6 mV. The 3E system and 2E system showed the standard deviations of OCP of 0.0026 mV and 0.32 mV, respectively. It revealed that the EC 6-well plate with the 3E system is excellent for developing an EC immunosensor.