Kinesin-2 differentially regulates the anterograde axonal transports of acetylcholinesterase and choline acetyltransferase in Drosophila
Kinesin-2 differentially regulates the anterograde axonal transports of acetylcholinesterase and choline acetyltransferase in Drosophila
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DOI：
10.1002/neu.20230
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发表时间：
2006-03-01
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影响因子：
--
通讯作者：
Ray, K
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文献类型：
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作者：
Baqri, R;Charan, R;Ray, K
胆碱乙酰转移酶（ChAT）和乙酰胆碱酯酶（AChE）分别参与突触前和突触后区室中乙酰胆碱的合成和降解。在这里，我们表明，它们在果蝇幼虫神经节的顺行运输是微管依赖性的，并发生在两个不同的时间prof iles。在三龄幼虫期，AChE的运输是组成性的，而ChAT的运输是短暂的脉冲式的。驱动蛋白-2马达亚基Klp 64 D的突变和所有三个驱动蛋白-2亚基的单独siRNA介导的敲除破坏ChAT和AChE转运，并且这些抗原在神经元细胞体和轴突中的离散非重叠斑点中积累。定量分析进一步表明Klp 64 D突变可以独立影响AChE的顺行转运，甚至在ChAT之前。最后，ChAT和AChE与驱动蛋白-2亚基共免疫沉淀，但彼此不共沉淀。总之，这些表明，驱动蛋白-2独立运输AChE和ChAT在同一轴突。这也意味着货物的可用性可以调节驱动蛋白马达的运输速率和频率。(c)2006 Wiley Periodicals，Inc.
Choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) are involved in acetylcholine synthesis and degradation at pre- and postsynaptic compartments, respectively. Here we show that their anterograde transport in Drosophila larval ganglion is microtubule-dependent and occurs in two different time prof iles. AChE transport is constitutive while that of ChAT occurs in a brief pulse during third instar larva stage. Mutations in the kinesin-2 motor subunit Klp64D and separate siRNA-mediated knockouts of all the three kinesin-2 subunits disrupt the ChAT and AChE transports, and these antigens accumulate in discrete nonover-lapping punctae in neuronal cell bodies and axons. Quantification analysis further showed that mutations in Klp64D could independently affect the anterograde transport of AChE even before that of ChAT. Finally, ChAT and AChE were coimmunoprecipitated with the kinesin-2 subunits but not with each other. Altogether, these suggest that kinesin-2 independently transports AChE and ChAT within the same axon. It also implies that cargo availability could regulate the rate and frequency of transports by kinesin motors. (c) 2006 Wiley Periodicals, Inc.