Terminal Differentiation of Human Promyelocytic Leukemic Cells in Primary Culture in Response to Retinoic Acid
Terminal Differentiation of Human Promyelocytic Leukemic Cells in Primary Culture in Response to Retinoic Acid
复制标题
DOI：
10.1182/blood.v57.6.1000.bloodjournal5761000
复制
发表时间：
1981-06
期刊：
影响因子：
20.3
通讯作者：
T. Breitman;S. Collins;B. Keene
中科院分区：
文献类型：
--
作者：
T. Breitman;S. Collins;B. Keene
最近的发现，视黄酸诱导人类早幼粒细胞白血病细胞系HL-60的终末粒细胞分化，促使在原代悬浮培养的人髓细胞白血病细胞对这种诱导剂的敏感性的调查。在21例白血病标本中，只有两例急性早幼粒细胞白血病患者的细胞对维甲酸有反应。在1 μ M视黄酸中孵育5- 7天后，来自这两名患者的细胞显示出广泛的形态和功能成熟。因此，由于视黄酸似乎特异性诱导白血病早幼粒细胞的粒细胞分化，因此该化合物可能在治疗急性早幼粒细胞白血病中具有治疗效用。
The recent finding that retinoic acid induces terminal granulocytic differentiation of the human promyelocytic leukemia cell line, HL-60, prompted an investigation of the sensitivity to this inducer of human myelocytic leukemia cells in primary suspension culture. Of the 21 leukemic specimens, only cells from the two patients with acute promyelocytic leukemia differentiated in response to retinoic acid. After an incubation period of 5--7 days in 1 microM retinoic acid, the cells from these two patients showed extensive morphological and functional maturation. Thus, because it appears that retinoic acid specifically induces granulocytic differentiation of leukemic promyelocytes, this compound may have therapeutic utility in the treatment of acute promyelocytic leukemia.