Neutrophil elastase and myeloperoxidase mRNA expression in overweight and obese subjects
Neutrophil elastase and myeloperoxidase mRNA expression in overweight and obese subjects
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DOI：
10.1007/s11033-018-4279-4
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发表时间：
2018-10-01
影响因子：
2.8
通讯作者：
Biswas, Subrata K.
中科院分区：
文献类型：
--
作者：
Ali, Mohammad;Jasmin, Shahana;Biswas, Subrata K.
在动物模型中，神经弹性蛋白酶和髓过氧化物酶与高脂饮食诱导的肥胖、胰岛素抵抗（IR）和动脉粥样硬化有关。本研究旨在探讨超重和肥胖患者外周血白细胞（PBL）中性粒细胞弹性蛋白酶和髓过氧化物酶mRNA的表达，并探讨其与BMI、IR和心血管生物标志物的相关性。在这项横断面研究中，招募了74名明显健康的受试者，包括22名瘦，27名超重和25名肥胖受试者。从空腹血液样本中评价心血管和代谢生物标志物。通过实时PCR定量PBL中中性粒细胞弹性蛋白酶和髓过氧化物酶基因的mRNA水平。与瘦型组相比，超重组和肥胖组PBL中中性粒细胞弹性蛋白酶（p < 0.001）和髓过氧化物酶（p < 0.03）mRNA表达均显著上调。但超重组和肥胖组之间无差异。中性粒细胞弹性蛋白酶和髓过氧化物酶mRNA水平与BMI、血清甘油三酯、血浆致动脉粥样硬化指数和10年心血管疾病发病风险呈显著正相关。但没有发现与葡萄糖，胰岛素或IR的相关性。它的结论是，中性粒细胞弹性蛋白酶和髓过氧化物酶基因在超重和肥胖的受试者上调，并与BMI和心血管疾病的标志物。
Neutrophil elastase and myeloperoxidase enzymes have been implicated in high-fat diet-induced obesity, insulin resistance (IR) and atherosclerosis in animal models. The aim of the present study was to explore neutrophil elastase and myeloperoxidase mRNA expressions in the peripheral blood leukocytes (PBL) in overweight and obese subjects, and to correlate those mRNA expressions with BMI, IR and cardiovascular biomarkers. In this cross-sectional study, 74 apparently healthy subjects including 22 lean, 27 overweight and 25 obese subjects were recruited. Cardiovascular and metabolic biomarkers were evaluated from fasting blood samples. The mRNA levels of neutrophil elastase and myeloperoxidase genes in the PBL were quantified by real-time PCR. Compared to lean group, the overweight and obese groups showed significant upregulation of both neutrophil elastase (p < 0.001) and myeloperoxidase (p < 0.03) mRNA expressions in the PBL. But no difference was found between overweight and obese groups. The neutrophil elastase and myeloperoxidase mRNA levels showed significant positive correlation with BMI, serum triglyceride, atherogenic index of plasma and 10-year risk of developing cardiovascular disease. But no correlation was found with glucose, insulin or IR. It was concluded that the neutrophil elastase and myeloperoxidase genes are up-regulated in both overweight and obese subjects and are associated with BMI and markers of cardiovascular disease.