The presence of aflatoxin B1-FAPY adduct and human papilloma virus in cervical smears from cancer patients in Mexico
The presence of aflatoxin B1-FAPY adduct and human papilloma virus in cervical smears from cancer patients in Mexico
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DOI：
10.1080/19440049.2011.647098
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发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Guardado-Estrada, Mariano
中科院分区：
文献类型：
--
作者：
Carvajal, Magda;Berumen, Jaime;Guardado-Estrada, Mariano
对40例宫颈癌患者和14例健康妇女宫颈刮片中的致癌标志物黄曲霉毒素B-1-甲酰胺基嘧啶2，3-dihydro-2-(N-formyl)-2‘，5’，6‘-triamino-4’-4‘-oxy-N-pyrimidyl-3-hydroxy-AFB(1)(1)-FAPY加合物和人乳头瘤病毒16型和18型进行了定量检测。确定了AFB(1)-Fapy加合物与HPV 16、18型之间的关系。竞争抑制间接酶联免疫吸附试验检测AFB(1)-Fapy加合物的抑制率为94%，定量检测限为0.1pg/mg，定量限为10pg/mg。癌组织中AFB(1)-Fapy加合物/mg DNA的平均浓度为1025pg，HPV16型为1420pg，HPV18型为630pg(p=0.03)。相比之下，健康对照组的
The carcinogenic biomarker aflatoxin B-1-formamidopyrimidine 2,3-dihydro-2-(N-formyl)-2',5',6'-triamino-4'-4'-oxy-N-pyrimidyl-3-hydroxy-AFB(1) called AFB(1)-FAPY adduct, and Human Papilloma Virus (HPV) types 16 and 18 were quantified from DNA cervical scrapes from 40 women with cervical cancer (CC) and 14 healthy women as controls. The relationship between the AFB(1)-FAPY adduct and HPV types 16 and 18 was determined. Competitive inhibitory indirect ELISA was validated with 94% inhibition to quantify the AFB(1)-FAPY adducts in picograms per milligram of DNA (limit of detection = 0.1 pg/mg, and limit of quantification = 10 pg/mg), polymerase chain reaction and DNA sequencing to identify HPV types. The average concentration of AFB(1)-FAPY adducts/mg DNA in the CC cases was 1025 pg, 1420 pg with HPV16 and 630 pg sharing HPV18 (p = 0.03). In comparison, healthy controls had