Candidate genes involving in tumorigenesis of cholangiocarcinoma induced by Opisthorchis viverrini infection
Candidate genes involving in tumorigenesis of cholangiocarcinoma induced by Opisthorchis viverrini infection
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DOI：
10.1007/s00436-011-2298-3
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发表时间：
2011-03
影响因子：
2
通讯作者：
Zhiliang Wu;T. Boonmars;S. Boonjaraspinyo;I. Nagano;S. Pinlaor;A. Puapairoj;P. Yongvanit;Y. Takahashi
中科院分区：
文献类型：
--
作者：
Zhiliang Wu;T. Boonmars;S. Boonjaraspinyo;I. Nagano;S. Pinlaor;A. Puapairoj;P. Yongvanit;Y. Takahashi
与后吸虫病相关的胆管癌（CCA）是阿后吸虫病流行地区的主要公共卫生问题之一。尽管CCA的流行与寄生虫感染之间的明确关系已被证明，但肿瘤发生的分子机制仍不清楚。本研究利用阿片吸虫病相关CCA动物模型，进行cDNA微阵列动力学分析，筛选参与阿片吸虫病相关CCA发生的候选基因。微阵列分析显示，CCA发生过程中有131个基因表达上调，包括与细胞增殖、分化和转化、细胞生长和周期调控、细胞凋亡、DNA修复和细胞骨架结构相关的基因。 145个基因表达下调，包括代谢酶、抑癌、凋亡、氧化反应和氧化还原相关基因。本研究列出了与肿瘤发生有关的候选基因，提供了阿后吸虫病相关CCA发生的分子信息以及诊断和治疗的潜在生物标志物，并提示细胞分化、增殖、转化相关基因的表达增加和代谢酶表达的减少可能在CCA的肿瘤发生中发挥重要作用。
Opisthorchiasis-associated cholangiocarcinoma (CCA) is one of main public health problems inOpisthorchis viverriniendemic areas. Although the definite relationship between prevalence of CCA and the parasite infection has been demonstrated, the molecular mechanism of tumorigenesis is still unknown. In the present study, by using animal model of opisthorchiasis-associated CCA, a kinetic analysis of cDNA microarray was performed to screen the candidate genes that involve in the development of opisthorchiasis-associated CCA. Microarray analysis revealed that the expressions of 131 genes were up-regulated during the development of CCA, including the genes relative to cell proliferation, differentiation and transformation, cell growth and cycle regulation, apoptosis, DNA repair, and cytoskeletal structure. The expressions of 145 genes were down-regulated, including the genes relative to metabolic enzymes, tumor suppressor, apoptosis, and oxidative response and oxidation reduction. The present study listed up the candidate genes involving tumorigenesis, provided molecular information on the development of opisthorchiasis-associated CCA and the potential biomarkers for diagnosis and therapy, and suggested that the increased expression of cell differentiation, proliferation, transformation-related genes, and decreased expression of metabolic enzymes may play important roles in the tumorigenesis of CCA.