Intranasal administration of Lactobacillus rhamnosus GG protects mice from H1N1 influenza virus infection by regulating respiratory immune responses
Intranasal administration of Lactobacillus rhamnosus GG protects mice from H1N1 influenza virus infection by regulating respiratory immune responses
复制标题
DOI：
10.1111/j.1472-765x.2010.02844.x
复制
发表时间：
2010-06-01
影响因子：
2.4
通讯作者：
Yausi, H.
中科院分区：
文献类型：
--
作者：
Harata, G.;He, F.;Yausi, H.
目的：研究鼻内乳酸杆菌给药是否通过增强小鼠模型呼吸道免疫应答来保护宿主动物免受流感病毒（IFV）感染。方法和结果：鼻内暴露于鼠李糖乳杆菌GG（LGG）3天后，BALB/c小鼠感染IFV A/PR/8/34（H1N1）。与对照组小鼠相比，LGG治疗组小鼠表现出较低的累积症状频率和较高的存活率（P < 0，中心点0.05）。从用LGG处理的小鼠分离的肺细胞的YAC-1细胞杀伤活性显著大于从对照小鼠分离的那些（P < 0，中心点01）。鼻内给予LGG显著增加白细胞介素（IL）-1 β、肿瘤坏死因子（TNF）和单核细胞趋化蛋白（MCP）-1的mRNA表达结论：这些结果表明，鼻内施用LGG通过在上调肺自然杀伤（NK）细胞活化后增强呼吸细胞介导的免疫应答来保护宿主动物免受IFV感染。我们已经证明，益生菌可以通过刺激呼吸道的免疫反应来保护宿主动物免受病毒感染。
Aims:To investigate whether intranasal Lactobacillus administration protects host animals from influenza virus (IFV) infection by enhancing respiratory immune responses in a mouse model.Methods and Results:After 3 days of intranasal exposure to Lactobacillus rhamnosus GG (LGG), BALB/c mice were infected with IFV A/PR/8/34 (H1N1). Mice treated with LGG showed a lower frequency of accumulated symptoms and a higher survival rate than control mice (P < 0 center dot 05). The YAC-1 cell-killing activity of lung cells isolated from mice treated with LGG was significantly greater than those isolated from control mice (P < 0 center dot 01). Intranasal administration of LGG significantly increased mRNA expression of interleukin (IL)-1 beta, tumour necrosis factor (TNF) and monocyte chemotactic protein (MCP)-1 (P < 0 center dot 01).Conclusions:These results suggest that intranasal administration of LGG protects the host animal from IFV infection by enhancing respiratory cell-mediated immune responses following up-regulation of lung natural killer (NK) cell activation.Significance and Impact of Study:We have demonstrated that probiotics might protect host animals from viral infection by stimulating immune responses in the respiratory tract.