Diversity in the Strength and Structure of Unruptured Cerebral Aneurysms.
Diversity in the Strength and Structure of Unruptured Cerebral Aneurysms.
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DOI：
10.1007/s10439-015-1252-4
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发表时间：
2015-07
影响因子：
3.8
通讯作者：
Cebral JR
中科院分区：
文献类型：
--
作者：
Robertson AM;Duan X;Aziz KM;Hill MR;Watkins SC;Cebral JR
颅内动脉瘤是脑动脉的病理性增大，被认为是由进行性壁退化和重塑引起的。早期使用经典组织学方法的工作确定了脑动脉瘤壁内容的变异性，从具有完整内皮和对齐平滑肌细胞的分层壁到薄的、细胞少的壁。在这里，我们利用多光子显微镜的最新进展，为 15 个未经染色或固定的人类动脉瘤圆顶以及 12 个对照脑动脉中的胶原纤维结构提供了新的结果。对于所有动脉瘤样本，弹性层不存在，腔外胶原纤维具有与控制动脉相似的直径。相比之下，腔侧的胶原纤维在直径和结构上表现出很大的变化，从致密的纤维层到稀疏的纤维结构，表明重塑工作无效。使用单轴实验评估了八个动脉瘤样品的机械完整性，揭示了两个子类（i）脆弱的未破裂动脉瘤（低失效应力和失效压力），和（ii）强未破裂动脉瘤（高失效应力和失效压力）。这些结果表明需要完善风险评估研究的终点，目前这些研究并未区分未破裂动脉瘤的风险水平。我们建议，识别这种脆弱的壁亚群的壁完整性测量方法将有助于解释未来的生物和结构数据。
Intracranial aneurysms are pathological enlargements of brain arteries that are believed to arise from progressive wall degeneration and remodeling. Earlier work using classical histological approaches identified variability in cerebral aneurysm mural content, ranging from layered walls with intact endothelium and aligned smooth muscle cells, to thin, hypocellular walls. Here, we take advantage of recent advances in multiphoton microscopy, to provide novel results for collagen fiber architecture in 15 human aneurysm domes without staining or fixation as well as in 12 control cerebral arteries. For all aneurysm samples, the elastic lamina was absent and the abluminal collagen fibers had similar diameters to control arteries. In contrast, the collagen fibers on the luminal side showed great variability in both diameter and architecture ranging from dense fiber layers to sparse fiber constructs suggestive of ineffective remodeling efforts. The mechanical integrity of eight aneurysm samples was assessed using uniaxial experiments, revealing two sub-classes (i) vulnerable unruptured aneurysms (low failure stress and failure pressure), and (ii) strong unruptured aneurysms (high failure stress and failure pressure). These results suggest a need to refine the end-point of risk assessment studies that currently do not distinguish risk levels among unruptured aneurysms. We propose that a measure of wall integrity that identifies this vulnerable wall subpopulation will be useful for interpreting future biological and structural data.
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影响因子：
4.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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