Characteristics of a pandemic clone of O3: K6 and O4: K68 Vibrio parahaemolyticus isolated in Beira, Mozambique
Characteristics of a pandemic clone of O3: K6 and O4: K68 Vibrio parahaemolyticus isolated in Beira, Mozambique
复制标题
DOI：
10.1099/jmm.0.2008/004275-0
复制
发表时间：
2008-12-01
影响因子：
3
通讯作者：
Kim, Dong Wook
中科院分区：
文献类型：
--
作者：
Ansaruzzaman, M.;Chowdhury, Ashrafuzzaman;Kim, Dong Wook
本研究对2004年和2005年在莫桑比克分离的副溶血性弧菌的遗传特性进行了评估，以确定副溶血性弧菌O3：K6和O4：K68血清型的大流行克隆是否已传播到莫桑比克。对从莫桑比克贝拉住院腹泻患者中分离的58株副溶血性弧菌进行O：K抗原血清学分型，并对毒素R、tdh和trh基因进行分型。用群特异性聚合酶链式反应、检测丝状噬菌体F237 ORF8的聚合酶链式反应、随机引物式聚合酶链式反应、脉冲场凝胶电泳法和多位点分型来确定菌株及其祖先的流行状态。通过这些分析，所有的O3：K6(n=38)和O4：K68(n=4)血清型都被鉴定为大流行克隆群。这些毒株与大流行参考毒株臭氧：K6和O4：K68密切相关，这两种毒株于1996年在亚洲出现，后来在全球发现。大流行血清型O3：K6和O4：K68，包括参考毒株，归入一个单独的簇，表明从共同的祖先出现。O_3：K_(58)(n=8)、O_4：K_(13)(n=6)、O_3：KUT(n=1)和O_8：K_(41)(n=1)表现出不同于大流行克隆的独特特征。
The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V parahaemolyticus strains isolated from hospitalized diarrhoea patients in Beira, Mozambique, were serotyped for O : K antigens and genotyped for toxR, tdh and trh genes. A group-specific PCR, a PCR that detects the presence of ORF8 of the filamentous phage f237, arbitrarily primed PCR, PFGE and multilocus sequence typing were performed to determine the pandemic status of the strains and their ancestry. All strains of serovars O3 : K6 (n=38) and O4 : K68 (n=4) were identified as a pandemic clonal group by these analyses. These strains are closely related to the pandemic reference strains of O3 : K6 and O4 : K68, which emerged in Asia in 1996 and were later found globally. The pandemic serotypes O3 : K6 and O4 : K68 including reference strains grouped into a single cluster indicating emergence from a common ancestor. The O3 : K58 (n=8), O4 : K13 (n=6), O3 : KUT (n=1) and O8 : K41 (n=1) strains showed unique characteristics different from the pandemic clone.