Characteristics and regulatory pathway of the PrupeSEP1 SEPALLATA gene during ripening and softening in peach fruits
Characteristics and regulatory pathway of the PrupeSEP1 SEPALLATA gene during ripening and softening in peach fruits
复制标题
PrupeSEP1 SEPALLATA基因在桃果实成熟软化过程中的特性及调控途径
DOI：
10.1016/j.plantsci.2017.01.004
复制
发表时间：
2017-04-01
期刊：
影响因子：
5.2
通讯作者：
Zhao, Caiping
中科院分区：
文献类型：
--
作者：
Li, Jinjin;Fang, Li;Zhao, Caiping
SEPALLATA基因是MADS-box转录因子亚家族的成员，在花器官发育和果实成熟过程中发挥重要作用。本研究从桃肉中克隆了PrupeSEP1基因。其推导的氨基酸序列与苹果中的MdMADS8和MdMADS9以及草莓中的MADS-RIN非常相似。在融肉桃贮藏过程中，SEP1的表达模式与乙烯生物合成和乙烯信号转导相关基因(EIN2和ETR2)的表达模式相似。SEP1表达水平与EIN2、ETR2表达水平呈正相关。此外，在MF中，与细胞壁修饰相关的基因(Endo-PG3、EXP2和PME3)、N-糖链加工基因(β-Hex2和α-Man)和Lox1显示出与SEP1相似的表达模式。然而，在非融化肉(NMF)桃中，SEP1的表达模式与非融化桃不同。此外，在NMF中，只有EXP2、LOX1和α-Man的表达模式与SEP1相似。用病毒诱导的基因沉默技术(VIGS)抑制SEP1的表达后，与对照相比，果实保持坚硬，果实软化延缓。与果实成熟和软化相关的基因ACS2、EIN2、PME1、Endo-PG3、ACOI、ETR2、β-Hex2和Lox1的表达水平显著降低，而EXP2和PME3的转录水平除采收期外显著增强。酵母单杂交验证表明，SEP1能与靶基因PGs的启动子相互作用。我们的结果表明，SEP1可能调节了MF桃的果实成熟和软化，而NMF桃则可能失去了这一调节。(C)2017爱思唯尔B.V.保留所有权利。
SEPALLATA genes are members of a subfamily of MADS-box transcription factors, and have essential roles in floral organ development and fruit ripening. In this study, the PrupeSEP1 gene was cloned from peach flesh. Its deduced amino acid sequence was very similar to that of MdMADS8 and MdMADS9 in apple and MADS-RIN-like in strawberry. During storage of melting flesh (MF) peach, the pattern of SEP1 expression was similar to that of ethylene biosynthesis and ethylene signal transduction-related gene expression (EIN2 and ETR2). The SEP1 expression level was correlated with that of EIN2 and ETR2. Furthermore, in MF, cell wall modification-related genes (Endo-PG3, EXP2 and PME3), N-glycan processing genes (beta-Hex2 and alpha-Man) and Lox1 exhibited similar expression patterns to that of SEP1. However, in non-melting flesh (NMF) peach, the SEP1 expression pattern was different from that of MF peach. Moreover, in NMF, only EXP2, LOX1, and alpha-Man expression patterns were similar to that of SEP1. After SEP1 expression was inhibited by virus-induced gene silencing technique (VIGS) in MF peach, compared with the control, the fruit remained firm and fruit softening was delayed. While expression levels of the ripening and softening related genes, ACS2, EIN2, PME1, Endo-PG3, ACOI, ETR2, beta-Hex2 and Lox1, were significantly decreased in SEP1-silenced peach, the transcription of EXP2 and PME3 were significantly enhanced except at the harvest stage. Yeast one-hybrid verification showed SEP1 can interact with promoter of target genes PGs. Our results indicate SEP1 may regulate fruit ripening and softening of MF peaches, while this regulation may be lost in the NMF peaches. (C) 2017 Elsevier B.V. All rights reserved.