A protein phosphatase 2A complex spatially controls plant cell division
A protein phosphatase 2A complex spatially controls plant cell division
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DOI：
10.1038/ncomms2831
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发表时间：
2013-05-01
影响因子：
16.6
通讯作者：
Pastuglia, Martine
中科院分区：
文献类型：
--
作者：
Spinner, Lara;Gadeyne, Astrid;Pastuglia, Martine
在没有细胞迁移的情况下，细胞分裂的方向对于植物体平面的确定至关重要。分裂平面在植物细胞中的位置是有丝分裂前通过一个短暂的细胞骨架阵列，preprophase带，它精确地描绘了分裂的皮层平面。在这里，我们描述了一种蛋白质复合物，目标蛋白磷酸酶2A活性的微管，调节过渡从间期到有丝分裂前的微管阵列。该复合物包含TONNEAU 1和具有FASS作为调节亚基的PP 2A异源三聚体全酶，通过TONNEAU 1募集基序蛋白家族募集到细胞骨架。尽管acentrosomal性质的植物细胞，这个复杂的所有成员共享相似性与动物中心体蛋白质参与纤毛和中心粒/中心体功能，揭示了植物细胞的皮质细胞骨架和微管组织者在其他真核生物之间的进化联系。
In the absence of cell migration, the orientation of cell divisions is crucial for body plan determination in plants. The position of the division plane in plant cells is set up premitotically via a transient cytoskeletal array, the preprophase band, which precisely delineates the cortical plane of division. Here we describe a protein complex that targets protein phosphatase 2A activity to microtubules, regulating the transition from the interphase to the premitotic microtubule array. This complex, which comprises TONNEAU1 and a PP2A heterotrimeric holoenzyme with FASS as regulatory subunit, is recruited to the cytoskeleton via the TONNEAU1-recruiting motif family of proteins. Despite the acentrosomal nature of plant cells, all members of this complex share similarity with animal centrosomal proteins involved in ciliary and centriolar/centrosomal functions, revealing an evolutionary link between the cortical cytoskeleton of plant cells and microtubule organizers in other eukaryotes.