Cell surface N-glycans influence the level of functional E-cadherin at the cell-cell border
Cell surface N-glycans influence the level of functional E-cadherin at the cell-cell border
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DOI：
10.1016/j.fob.2014.10.006
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发表时间：
2014-01-01
期刊：
影响因子：
2.6
通讯作者：
Schwalbe, Ruth A.
中科院分区：
文献类型：
--
作者：
Hall, M. Kristen;Weidner, Douglas A.;Schwalbe, Ruth A.
E-钙粘附素对细胞之间的黏附至关重要，从而对组织的发育和维持至关重要。虽然已经证实细胞内的N-葡聚糖影响上皮源性细胞表面E-钙粘附素的水平，但尚不清楚细胞外的N-葡聚糖是否控制E-钙粘附素在细胞-细胞边界的聚集。在这里，我们证明了细胞表面N-糖链的减少削弱了E-钙粘附素在细胞-细胞边界的募集和保留，从而降低了细胞-细胞相互作用的强度。我们得出结论，细胞表面的N-糖链与E-钙粘附素在细胞-细胞边界的放置紧密相连，从而控制E-钙粘附素介导的细胞-细胞黏附。(C)2014年提交人。由Elsevier B.V.代表欧洲生化学会联合会出版。
E-cadherin is crucial for adhesion of cells to each other and thereby development and maintenance of tissue. While it is has been established that N-glycans inside the cell impact the level of E-cadherin at the cell surface of epithelial-derived cells, it is unclear whether N-glycans outside the cell control the clustering of E-cadherin at the cell-cell border. Here, we demonstrate reduction of N-glycans at the cell surface weakened the recruitment and retention of E-cadherin at the cell-cell border, and consequently reduced the strength of cell-cell interactions. We conclude that N-glycans at the cell surface are tightly linked to the placement of E-cadherin at the cell-cell border and thereby control E-cadherin mediated cell-cell adhesion. (C) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.