TECHNICAL REPORT - A NEW CHAMBER TECHNIQUE FOR MICRO-VASCULAR STUDIES IN UNANESTHETIZED HAMSTERS
TECHNICAL REPORT - A NEW CHAMBER TECHNIQUE FOR MICRO-VASCULAR STUDIES IN UNANESTHETIZED HAMSTERS
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DOI：
10.1007/bf01851841
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
MESSMER, K
中科院分区：
文献类型：
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作者：
ENDRICH, B;ASAISHI, K;MESSMER, K
设计了一个实验模型，用于直接、定量研究微循环中的血流动力学和形态参数。它包括在仓鼠背侧皮瓣中植入改良的阿尔吉室，并在颈静脉和颈动脉中植入 2 根永久性导管。使用活体显微镜和电视技术对血细胞速度和血管直径进行原位测量来研究微循环。由于血细胞、毛细血管和周围皮下组织之间的对比度较差。静脉注射后使用荧光显微镜观察组织、微血管床。注射 0.2 ml 5% FITC[异硫氰酸荧光素]-右旋糖酐 150。光学元件和少量 FITC-右旋糖酐的组合提高了电视图像的对比度，而不改变宏观和微观血液动力学参数，并且血浆在红细胞和周围组织的较暗背景下被描绘为明亮的结构。这使得可以对给定皮下区域内的几乎所有血管进行定量研究。未麻醉动物的组织。小动脉中的血细胞速度为0.7-1.1毫米/秒，毛细血管中部为0.2-0.7毫米/秒，集合小静脉中的血细胞速度达到0.6毫米/秒。自从静脉注射以来该模型可以进行药物注射和全身压力测量，它为研究长期微循环的反应性提供了独特的方法。
An experimental model was designed for direct, quantitative studies of hemodynamic and morphologic parameters in the microcirculation. It consists of implanting a modified Algire chamber in the dorsal skin flap of hamsters and the implementation of 2 permanent catheters in jugular vein and carotid artery. The microcirculation was studied using intravital microscopy and television techniques for in situ measurements of blood cell velocity and vascular diameters. Due to the poor contrast between blood cells, blood capillaries and surrounding s.c. tissue, microvascular beds were visualized using fluorescent microscopy after i.v. injection of 0.2 ml of 5% FITC[fluorescein isothiocyanate]-Dextran 150. The combination of optical elements and low amounts of FITC-Dextran improved the contrast of the televised image without changing macro- and microhemodynamic parameters, and blood plasma was delineated as bright structure against the substantially darker background of red blood cells and surrounding tissue. This permitted the quantitative study of practically all blood vessels within a given field of s.c. tissue in unanesthetized animals. Blood cell velocity in arterioles was 0.7-1.1 mm/s, 0.2-0.7 mm/s in midcapillaries and reached 0.6 mm/s in collecting venules. Since i.v. injection of drugs and systemic pressure measurements are possible in this model, it provides a unique means for studying the reactivity of the microcirculation over a prolonged period.