Characterization of a Chlamydomonas transposon, Gulliver, resembling those in higher plants.
Characterization of a Chlamydomonas transposon, Gulliver, resembling those in higher plants.
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衣藻转座子 Gulliver 的表征，类似于高等植物中的转座子。
DOI：
10.1093/genetics/122.2.363
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发表时间：
1989
期刊：
影响因子：
3.3
通讯作者：
Ferris,PJ
中科院分区：
文献类型：
--
作者：
Ferris,PJ
在对莱茵衣藻连锁群VI的mt+位点进行染色体步移时，我发现一个12 kb的序列在核基因组中大约有12个拷贝。不同品种C. Reinhardtii实验室菌株提供了该序列是移动的并因此是转座子的证据。两个独立的自然分离物之一，可与C。reinhardtii，C. smithii（CC-1373）含有转座子，但在其核基因组中的位置与C.第二个，CC-1952（S1-C5），完全缺乏转座子。遗传分析表明，转座子被发现在整个基因组中分散的位点，但在每个位置有一个保守的结构。末端之间的序列同源性仅限于不完美的15-bp反向重复。通过插入产生8-bp靶位点重复;在切除后完全去除转座子序列，留下靶位点重复的两个拷贝，具有微小的碱基变化。该转座子含有一个独特的重复序列的内部区域，负责限制性片段长度的异质性之间的转座子的各种拷贝。在一些情况下，当转座事件发生时，可以确定给定菌株中的十几个转座子中的哪一个作为供体。转座子经常移动到与供体基因相连的位点，并且转座似乎是非复制性的。因此，我命名为格列佛的转座子的转座和切除机制类似于某些高等植物转座子，如玉米的Ac转座子。
While pursuing a chromosomal walk through the mt+ locus of linkage group VI of Chlamydomonas reinhardtii, I encountered a 12-kb sequence that was found to be present in approximately 12 copies in the nuclear genome. Comparison of various C. reinhardtii laboratory strains provided evidence that the sequence was mobile and therefore a transposon. One of two separate natural isolates interfertile with C. reinhardtii, C. smithii (CC-1373), contained the transposon, but at completely different locations in its nuclear genome than C. reinhardtii; and a second, CC-1952 (S1-C5), lacked the transposon altogether. Genetic analysis indicated that the transposon was found at dispersed sites throughout the genome, but had a conserved structure at each location. Sequence homology between the termini was limited to an imperfect 15-bp inverted repeat. An 8-bp target site duplication was created by insertion; transposon sequences were completely removed upon excision leaving behind both copies of the target site duplication, with minor base changes. The transposon contained an internal region of unique repetitive sequence responsible for restriction fragment length heterogeneity among the various copies of the transposon. In several cases it was possible to identify which of the dozen transposons in a given strain served as the donor when a transposition event occurred. The transposon often moved into a site genetically linked to the donor, and transposition appeared to be nonreplicative. Thus the mechanism of transposition and excision of the transposon, which I have named Gulliver, resembles that of certain higher plant transposons, like the Ac transposon of maize.