Assessment of Full-Eye Response to Osmotic Stress in Mouse Model In Vivo Using Optical Coherence Tomography.
Assessment of Full-Eye Response to Osmotic Stress in Mouse Model In Vivo Using Optical Coherence Tomography.
复制标题
使用光学相干断层扫描评估体内小鼠模型对渗透应激的全眼反应
DOI：
10.1155/2015/568509
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发表时间：
2015
影响因子：
1.9
通讯作者：
Li P
中科院分区：
文献类型：
--
作者：
Ni Y;Xu B;Wu L;Du C;Jiang B;Ding Z;Li P
以NaCl为基础的溶液作为渗透应激剂，改变小鼠眼睛的水化状态。使用定制的光学相干断层扫描（OCT）监测全眼对这些渗透挑战的体内反应，其成像范围扩展到12.38 mm。基于OCT图像，使用角膜中央厚度（CCT）、前房深度（ACD）、晶状体厚度（LT）、角膜-视网膜距离（CRD）、虹膜曲率（IC）和晶状体散射强度（LSI）等参数量化小鼠眼睛的动态变化。几乎所有眼部成分的形态和晶状体的透光性都发生了明显但可逆的变化。特别是，高渗挑战引起的眼部脱水导致虹膜角膜角闭合和晶状体混浊。我们的研究结果表明，眼水合是一个重要的生理过程，可能与干眼、白内障、闭角型青光眼等多种眼部疾病有关，并会影响眼睛的生物测量和成像。OCT能够全面研究眼内水合反应的动态全眼反应。
NaCl based solutions were applied as osmotic stress agents to alter the hydration state of the mouse eye. Full-eye responses to these osmotic challenges were monitored in vivo using a custom-built optical coherence tomography (OCT) with an extended imaging range of 12.38 mm. Dynamic changes in the mouse eye were quantified based on the OCT images using several parameters, including the central corneal thickness (CCT), the anterior chamber depth (ACD), the crystalline lens thickness (LT), the cornea-retina distance (CRD), the iris curvature (IC), and the lens scattering intensity (LSI). Apparent but reversible changes in the morphology of almost all the ocular components and the light transparency of the lens are exhibited. Particularly, the ocular dehydration induced by the hypertonic challenges resulted in a closing of the iridocorneal angle and an opacification of the lens. Our results indicated that the ocular hydration is an important physiological process which might be correlated with various ocular disorders, such as dry eye, cataract, and angle-closure glaucoma, and would affect the biometry and imaging of the eye. OCT uniquely enables the comprehensive study of the dynamic full-eye responses to the ocular hydration in vivo.