MPK3/MPK6-mediated phosphorylation of ERF72 positively regulates resistance to Botrytis cinerea through directly and indirectly activating the transcription of camalexin biosynthesis enzymes
MPK3/MPK6-mediated phosphorylation of ERF72 positively regulates resistance to Botrytis cinerea through directly and indirectly activating the transcription of camalexin biosynthesis enzymes
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DOI：
10.1093/jxb/erab415
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发表时间：
2021-09-09
影响因子：
6.9
通讯作者：
Han, Shengcheng
中科院分区：
文献类型：
--
作者：
Li, Yihao;Liu, Kun;Han, Shengcheng
乙烯反应因子 (ERF) VII 组成员通常在调节拟南芥植物生长和发育、非生物胁迫反应和植物免疫方面发挥作用；然而，VII组ERF介导植物免疫反应的调节机制的细节仍然难以捉摸。在这里，我们将其中一个成员 ERF72 定性为介导对坏死性病原体灰葡萄孢 (Botrytis cinerea) 的抗性的正调节因子。与野生型（WT）相比，erf72突变体表现出较低的camalexin浓度并且对灰霉病菌更敏感，而erf72中ERF72的互补挽救了易感表型。此外，野生型中ERF72的过度表达促进了camalexin的生物合成并增加了对灰霉病菌的抗性。我们确定了camalexin生物合成基因PAD3和CYP71A13以及转录因子WRKY33作为ERF72的靶基因。我们还确定 MPK3 和 MPK6 在 Ser151 位点磷酸化 ERF72 并提高其反式激活活性，从而导致 camalexin 浓度增加和对 B. cinerea 的抗性增加。因此，ERF72在植物免疫中发挥作用，通过直接调节生物合成基因的表达和间接通过靶向WRKK33来协调camalexin生物合成。
Ethylene response factor (ERF) Group VII members generally function in regulating plant growth and development, abiotic stress responses, and plant immunity in Arabidopsis; however, the details of the regulatory mechanism by which Group VII ERFs mediate plant immune responses remain elusive. Here, we characterized one such member, ERF72, as a positive regulator that mediates resistance to the necrotrophic pathogen Botrytis cinerea. Compared with the wild-type (WT), the erf72 mutant showed lower camalexin concentration and was more susceptible to B. cinerea, while complementation of ERF72 in erf72 rescued the susceptibility phenotype. Moreover, overexpression of ERF72 in the WT promoted camalexin biosynthesis and increased resistance to B. cinerea. We identified the camalexinbiosynthesis genes PAD3 and CYP71A13 and the transcription factor WRKY33 as target genes of ERF72. We also determined that MPK3 and MPK6 phosphorylated ERF72 at Ser151 and improved its transactivation activity, resulting in increased camalexin concentration and increased resistance to B. cinerea. Thus, ERF72 acts in plant immunity to coordinate camalexin biosynthesis both directly by regulating the expression of biosynthetic genes and indirectly by targeting WRKK33.