Coupling cell shape and velocity leads to oscillation and circling in keratocyte galvanotaxis
Coupling cell shape and velocity leads to oscillation and circling in keratocyte galvanotaxis
复制标题
细胞形状和速度的耦合导致角膜细胞趋电性的振荡和循环
DOI：
10.1016/j.bpj.2022.11.021
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发表时间：
2023
影响因子：
3.4
通讯作者：
Camley, Brian A.
中科院分区：
文献类型：
--
作者：
Nwogbaga, Ifunanya;Camley, Brian A.
在伤口愈合期间，鱼角膜细胞经历趋电性，其中它们遵循伤口诱导的电场。除了常规的持续运动之外，角膜细胞可以在没有场的情况下进行圆周运动，或者在沿场方向爬行时振荡。我们开发了一个粗粒度的现象学模型，捕捉这些角膜细胞的行为。我们拟合这个模型的实验数据上的角膜细胞响应的电场被turnedon.A关键元素，我们的模型是一个倾向于细胞转向他们的长轴，所产生的细胞形状和速度之间的耦合，从而引起振荡和圆周运动。趋电性不仅受场依赖性反应的影响，还受细胞速度和细胞形状弛豫速率的影响。当细胞对打开的电场做出反应时，我们的模型预测僵硬，缓慢的细胞反应缓慢，但可靠地跟随信号。细胞以更快的速度与场结合并对齐，反应更快，更可靠地跟随信号。当细胞暴露于快速切换方向的场时，细胞遵循场方向的平均值，而如果场切换得更慢，则细胞遵循“阶梯”模式。我们的研究表明，一个简单的现象学模型耦合细胞的速度和形状是足够的再现各种各样的不同的角膜细胞的行为，从盘旋到振荡，以趋电反应，只有改变一些参数。
During wound healing, fish keratocyte cells undergo galvanotaxis where they follow a wound-induced electric field. In addition to their stereotypical persistent motion, keratocytes can develop circular motion without a field or oscillate while crawling in the field direction. We developed a coarse-grained phenomenological model that captures these keratocyte behaviors. We fit this model to experimental data on keratocyte response to an electric field being turned on. A critical element of our model is a tendency for cells to turn toward their long axis, arising from a coupling between cell shape and velocity, which gives rise to oscillatory and circular motion. Galvanotaxis is influenced not only by the field-dependent responses, but also cell speed and cell shape relaxation rate. When the cell reacts to an electric field being turned on, our model predicts that stiff, slow cells react slowly but follow the signal reliably. Cells that polarize and align to the field at a faster rate react more quickly and follow the signal more reliably. When cells are exposed to a field that switches direction rapidly, cells follow the average of field directions, while if the field is switched more slowly, cells follow a "staircase" pattern. Our study indicated that a simple phenomenological model coupling cell speed and shape is sufficient to reproduce a broad variety of different keratocyte behaviors, ranging from circling to oscillation to galvanotactic response, by only varying a few parameters.