A novel avian hypothalamic peptide inhibiting gonadotropin release
A novel avian hypothalamic peptide inhibiting gonadotropin release
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DOI：
10.1006/bbrc.2000.3350
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发表时间：
2000-08-28
影响因子：
3.1
通讯作者：
Sharp, JP
中科院分区：
文献类型：
--
作者：
Tsutsui, K;Saigoh, E;Sharp, JP
神经肽对垂体前叶水平促性腺激素分泌的控制主要是通过下丘脑十肽促性腺激素释放激素 (GnRH) 的刺激作用，GnRH 最初是从哺乳动物中分离出来的，后来又从非哺乳动物中分离出来。然而，迄今为止，脊椎动物中促性腺激素释放的抑制肽尚不清楚。在这里，我们在鸟类身上发现，下丘脑还含有一种抑制促性腺激素释放的新型肽。将鹌鹑脑的乙酸提取物通过 C-18 反相柱，然后将保留的物质进行反相和阳离子交换高效液相色谱 (HPLC)，从禽脑中分离出肽，其序列显示为 Ser-Ile-Lys-Pro-Ser-Ala-Tyr-Leu-Pro-Leu-Arg-Phe-NH2。含有该肽的细胞体和末端通过免疫组织化学方法分别定位于室旁核和正中隆起。这种肽以剂量相关的方式抑制培养的鹌鹑垂体前叶的促性腺激素释放。这是在脊椎动物中报道的第一个抑制促性腺激素释放的下丘脑肽。因此，我们将其称为促性腺激素抑制激素（GnIH）。 (C) 2000 年学术出版社。
The neuropeptide control of gonadotropin secretion at the level of the anterior pituitary gland is primarily through the stimulatory action of the hypothalamic decapeptide, gonadotropin-releasing hormone (GnRH), which was originally isolated from mammals and subsequently from nonmammals. To date, however, an inhibitory peptide of gonadotropin release is unknown in vertebrates. Here we show, in a bird, that the hypothalamus also contains a novel peptide which inhibits gonadotropin release. Acetic acid extracts of quail brains were passed through C-18 reversed-phase cartridges, and then the retained material was subjected to the reversed-phase and cation-exchange high-performance liquid chromatography (HPLC), The peptide was isolated from avian brain and shown to have the sequence Ser-Ile-Lys-Pro-Ser-Ala-Tyr-Leu-Pro-Leu-Arg-Phe-NH2. Cell bodies and terminals containing this peptide were localized immunohistochemically in the paraventricular nucleus and median eminence, respectively. This peptide inhibited, in a dose-related way, gonadotropin release from cultured quail anterior pituitaries. This is the first hypothalamic peptide inhibiting gonadotropin release reported in a vertebrate. We therefore term it gonadotropin-inhibitory hormone (GnIH). (C) 2000 Academic Press.