SPINAL-CORD ENERGY-METABOLISM FOLLOWING COMPRESSION TRAUMA TO THE FELINE SPINAL-CORD
SPINAL-CORD ENERGY-METABOLISM FOLLOWING COMPRESSION TRAUMA TO THE FELINE SPINAL-CORD
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DOI：
10.3171/jns.1980.53.3.0375
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发表时间：
1980-01-01
影响因子：
4.1
通讯作者：
SPEARS, CJ
中科院分区：
文献类型：
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作者：
ANDERSON, DK;MEANS, ED;SPEARS, CJ
猫脊髓受压创伤后 24 小时测定脊髓代谢状态。用戊巴比妥麻醉猫，并通过在脊髓上放置 190 克重物 5 分钟使其受伤。对受伤部位的生化分析显示，在整个 24 小时恢复期内，ATP、磷酸肌酸 (P-肌酸) 和总腺苷酸水平显着下降。血糖水平最初下降，但 1 小时后已恢复正常，并在 8 小时和 24 小时显着超正常。在损伤后的前 4 小时内，乳酸-丙酮酸比率和组织乳酸浓度分别增加了 4 倍和 5 1/2 倍。 8-24小时期间，乳酸水平保持升高，由于组织丙酮酸浓度显着升高，乳酸-丙酮酸比率下降至对照水平。整个受伤节段的代谢可能并不均匀，并且在创伤后的最初 4 小时内组织代谢率降低，然后在 24 小时恢复期的剩余时间内代谢活跃的组织代谢率增加。这种脊髓损伤模型会导致受影响组织严重、长期缺血和代谢损伤。这些代谢变化是否是由与此类脊髓损伤相关的组织损伤和不可逆性截瘫引起或导致仍有待确定。
The spinal cord metabolic state was determined for 24 h after compression trauma to the feline spinal cord. Cats were anesthetized with pentobarbital and injured by placing a 190 g weight on the spinal cord for 5 min. Biochemical analysis of the injured segment revealed a significant depletion in the levels of ATP, phosphocreatine (P-creatine) and total adenylates for the entire 24 h recovery period. Glucose levels initially declined but by 1 h had normalized and at 8 and 24 h were significantly supranormal. The lactate-pyruvate ratio and tissue lactate concentrations increased 4 and 5 1/2 times, respectively, for the first 4 h after injury. Between 8-24 h, lactate levels remained elevated, the lactate-pyruvate ratio declined to control levels as the result of a significant rise in the tissue pyruvate concentration. Metabolism was probably not homogeneous throughout the injured segment and the tissue metabolic rate was depressed for the initial 4 h after trauma, then increased in metabolically active tissue for the remainder of the 24 h recovery period. This model of spinal cord trauma results in a severe, prolonged ischemia and metabolic injury to the affected tissue. Whether these metabolic changes result from or cause the tissue damage and irreversible paraplegia associated with this type of spinal cord injury remains to be determined.