Toxicity and differential protein analysis following destruxin A treatment of Spodoptera litura (Lepidoptera: Noctuidae) SL-1 cells
Toxicity and differential protein analysis following destruxin A treatment of Spodoptera litura (Lepidoptera: Noctuidae) SL-1 cells
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destruxin A 处理斜纹夜蛾（鳞翅目：夜蛾科）SL-1 细胞后的毒性和差异蛋白分析
DOI：
10.1016/j.toxicon.2011.06.002
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发表时间：
2011-09-15
期刊：
影响因子：
2.8
通讯作者：
Ren, Shunxiang
中科院分区：
文献类型：
--
作者：
Meng, Xiang;Xu, Xiaoxia;Ren, Shunxiang
研究了破坏素A（DA）对斜纹夜蛾SL-1细胞的细胞毒性。MTT法显示DA对SL-1细胞有明显的毒性作用，并呈浓度和时间依赖性。DA处理24 h和48 h后的IC 50值分别为17.86 μ g/mL和7.80 μ g/mL。倒置相差显微镜（IPCM）观察发现，DA长时间处理可引起细胞变圆、细胞膜皱缩、凋亡小体形成、空泡出现和胞浆漏出。荧光显微镜和流式细胞术进一步证实了DA诱导的细胞凋亡。2.5 μ g/mL DA处理后SL-1细胞进入早期凋亡，随着DA浓度的增加，SL-1细胞进入晚期凋亡。此外，采用双向凝胶电泳（2-DE）分析鉴定了22个差异表达的蛋白质在对照细胞和DA处理的细胞之间，这些蛋白质的表达水平存在显著差异（>= 2倍差异），并且在处理的细胞和未处理的细胞之间，这些蛋白质的表达水平存在显著差异。我们的研究结果表明，这些差异表达的蛋白质可能有助于解释由destruxin A处理细胞引起的不同生物学效应;此外，一些鉴定的蛋白质可能在SL-1细胞增殖和凋亡中起作用。(C)2011年由Elsevier Ltd.出版
The cytotoxicity of a destruxin A (DA) treatment of Spodoptera litura SL-1 cells was investigated. An MTT assay showed that DA was highly toxic to SL-1 cells in a concentration- and time-dependent manner. The IC50 values of DA, after 24 h and 48 h of treatment, were 17.86 mu g/mL and 7.80 mu g/mL, respectively. Under inverted phase contrast microscopy (IPCM), it was found that prolonged treatment with DA could induce cell rounding, cellular membrane shrinking, formation of apoptotic bodies, vacuole appearance and cytoplasm leak out. Apoptosis induced by DA was further confirmed by fluorescence microscopy (FM) and flow cytometry (FCM) studies. SL-1 cells entered early apoptosis following a treatment with 2.5 mu g/mL DA and entered late apoptosis following a treatment with increasing concentrations of DA. Furthermore, two-dimensional gel electrophoresis (2-DE) analysis was used to identify 22 proteins which were differentially expressed (>= 2-fold difference) between control cells and DA-treated cells, and the expression level of these proteins was significantly different between the treated and untreated cells. Our results suggest that these differentially expressed proteins may help explain the diverse biological effects caused by the destruxin A treatment of cells; additionally, some of the identified proteins may have roles in SL-1 cellular proliferation and apoptosis. (C) 2011 Published by Elsevier Ltd.