In vitro free radical scavenging activity of platinum nanoparticles
In vitro free radical scavenging activity of platinum nanoparticles
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DOI：
10.1088/0957-4484/20/45/455105
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发表时间：
2009-11-11
期刊：
影响因子：
3.5
通讯作者：
Miyamoto, Yusei
中科院分区：
文献类型：
--
作者：
Watanabe, Aki;Kajita, Masashi;Miyamoto, Yusei
采用六氯铂酸盐醇还原法制备了聚丙烯酸（PAA）保护铂纳米粒子（PAA- pt）。PAA-Pt纳米颗粒分散均匀，平均粒径为2.0 +/- 0.4 nm （n = 200）。用电子自旋共振法定量了2,2-偶氮（2-氨基丙烷）二盐酸（AAPH）在O-2存在下热分解生成的过氧自由基（AOO（中心点）），用分光光度法定量了1,1-二苯基-2-吡啶酰肼（DPPH）自由基的残留。PAA-Pt以剂量依赖的方式清除这两种自由基。铂金是功能性成分。PAA-Pt降低了由AAPH产生的AOO（中心点）引发的亚油酸过氧化所需的耗氧量，表明抑制了亚油酸过氧化的繁殖。硫代巴比妥酸试验也显示了PAA-Pt对亚麻酸盐过氧化的剂量依赖性抑制作用。50微摩尔铂以PAA-Pt形式完全淬灭250 μ M DPPH自由基5分钟。即使将PAA-Pt稀释两倍，仍能100%淬灭250 μ M DPPH自由基。PAA-Pt的清除活性持久。这些观察结果表明PAA-Pt是一种有效的自由基清除剂。
A polyacrylic acid (PAA)-protected platinum nanoparticle species (PAA-Pt) was prepared by alcohol reduction of hexachloroplatinate. The PAA-Pt nanoparticles were well dispersed and homogeneous in size with an average diameter of 2.0 +/- 0.4 nm (n = 200). We used electron spin resonance to quantify the residual peroxyl radical (AOO(center dot)) generated from 2,2-azobis (2-aminopropane) dihydrochloride (AAPH) by thermal decomposition in the presence of O-2 and a spectrophotometric method to quantify the residual 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. PAA-Pt scavenged these two radicals in a dose-dependent manner. Platinum was the functional component. PAA-Pt reduced the rate of oxygen consumption required for linoleic acid peroxidation initiated by AOO(center dot) generated from AAPH, indicating inhibition of the propagation of linolate peroxidation. A thiobarbituric acid test also revealed dose-dependent inhibition of the linolate peroxidation by PAA-Pt. Fifty micromolar platinum, as PAA-Pt, completely quenched 250 mu M DPPH radical for 5 min. Even when twice diluted in half, the PAA-Pt still quenched 100% of the 250 mu M DPPH radical. The scavenging activity of PAA-Pt is durable. These observations suggest that PAA-Pt is an efficient scavenger of free radicals.