Transgenic tools to characterize neuronal properties of discrete populations of zebrafish neurons
Transgenic tools to characterize neuronal properties of discrete populations of zebrafish neurons
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DOI：
10.1242/dev.099531
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发表时间：
2013-09-15
期刊：
影响因子：
4.6
通讯作者：
Higashijima, Shin-ichi
中科院分区：
文献类型：
--
作者：
Satou, Chie;Kimura, Yukiko;Higashijima, Shin-ichi
发育中的神经系统由多种细胞类型组成。在特定种类的神经元细胞中表达报告基因的转基因动物是研究神经元网络形成的有力工具。我们产生了各种各样的转基因斑马鱼，在特定类别的神经元或神经元祖细胞中表达报告基因。这些包括特定神经递质表型的神经元表达荧光蛋白或Gal4的细胞系，以及脊髓中背侧祖细胞结构域的特定子集表达荧光蛋白的细胞系。利用这些，我们检查了发育中的脊髓背角的结构域组织，发现斑马鱼中有六个祖结构域，这与小鼠中的结构域组织相似。我们还系统地表征了从每个域产生的神经元的神经递质特性。鉴于报告基因的表达发生在广泛的区域的神经系统中产生的线，这些转基因鱼应该作为强大的工具，不仅在脊髓背侧的神经元，但也在许多其他地区的神经系统的神经元结构和功能的调查。
The developing nervous system consists of a variety of cell types. Transgenic animals expressing reporter genes in specific classes of neuronal cells are powerful tools for the study of neuronal network formation. We generated a wide variety of transgenic zebrafish that expressed reporter genes in specific classes of neurons or neuronal progenitors. These include lines in which neurons of specific neurotransmitter phenotypes expressed fluorescent proteins or Gal4, and lines in which specific subsets of the dorsal progenitor domain in the spinal cord expressed fluorescent proteins. Using these, we examined domain organization in the developing dorsal spinal cord, and found that there are six progenitor domains in zebrafish, which is similar to the domain organization in mice. We also systematically characterized neurotransmitter properties of the neurons that are produced from each domain. Given that reporter gene expressions occurs in a wide area of the nervous system in the lines generated, these transgenic fish should serve as powerful tools for the investigation of not only the neurons in the dorsal spinal cord but also neuronal structures and functions in many other regions of the nervous system.