Acidification prevents endothelial cell apoptosis by Axl activation
Acidification prevents endothelial cell apoptosis by Axl activation
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DOI：
10.1161/01.res.0000036753.50601.e9
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发表时间：
2002-10-04
影响因子：
20.1
通讯作者：
Capogrossi, MC
中科院分区：
文献类型：
--
作者：
D'Arcangelo, D;Gaetano, C;Capogrossi, MC
先前的研究表明，高碳引起的酸化可以保护内皮细胞免受血清剥夺诱导的凋亡。然而，酸化抗凋亡作用的机制尚不清楚。在5% CO2 （pH 7.4）和20% CO2 （pH 7.0）平衡的碳酸氢盐培养基中培养的人脐静脉内皮细胞进行cDNA阵列筛选。pH 7.0处理6 h后酪氨酸激酶受体Axl的表达量比pH 7.4高3.3倍；逆转录-聚合酶链反应证实了这种调节(3.0 +/- 0.9倍，P
Prior studies have shown that acidification due to hypercarbia protects endothelial cells from serum deprivation-induced apoptosis. However, the mechanism(s) responsible for the antiapoptotic effect of acidification is still unclear. cDNA array screening was performed on human umbilical vein endothelial cells cultured in a bicarbonate medium equilibrated either with 5% CO2 (pH 7.4) or with 20% CO2 (pH 7.0). Tyrosine kinase receptor Axl expression was 3.3-fold higher after 6 hours at pH 7.0 compared with pH 7.4; this modulation was confirmed by reverse transcriptase-polymerase chain reaction (3.0 +/- 0.9-fold, P