Suppression of human DNA alkylation-repair defects by Escherichia coli DNA-repair genes.
Suppression of human DNA alkylation-repair defects by Escherichia coli DNA-repair genes.
复制标题
大肠杆菌 DNA 修复基因抑制人类 DNA 烷基化修复缺陷。
DOI：
10.1073/pnas.83.15.5607
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Waldstein,EA
中科院分区：
文献类型：
--
作者：
Samson,L;Derfler,B;Waldstein,EA
ADA-alkB操纵子保护大肠杆菌免受许多烷化剂的影响。我们将其亚克隆到pSV2哺乳动物表达载体中，获得pSV2ada-alkB，并将其导入对烷化剂杀伤和姐妹染色单体交换非常敏感的Mer-HeLa S3细胞。其中一个转化子(S3-9细胞系)具有多个整合拷贝的pSV2ada-alkB，并被发现表达非常高水平的ada基因产物39-kDa O6-甲基鸟嘌呤-DNA甲基转移酶。S3-9细胞对两种烷基化试剂N-甲基-N‘-硝基-N-亚硝基和N，N’-双(2-氯乙基)-N-亚硝脲的杀伤和姐妹染色单体交换的诱导产生了抵抗力。这表明细菌DNA烷基化修复基因能够抑制人Mer细胞的烷基化修复缺陷。
The ada-alkB operon protects Escherichia coli against the effects of many alkylating agents. We have subcloned it into the pSV2 mammalian expression vector to yield pSV2ada-alkB, and this plasmid has been introduced into Mer- HeLa S3 cells, which are extremely sensitive to killing and induction of sister chromatid exchange by alkylating agents. One transformant (the S3-9 cell line) has several integrated copies of pSV2ada-alkB and was found to express a very high level of the ada gene product, the 39-kDa O6-methylguanine-DNA methyltransferase. S3-9 cells were found to have become resistant to killing and induction of sister chromatid exchange by two alkylating agents, N-methyl-N'-nitro-N-nitrosoguanidine and N,N'-bis(2-chloroethyl)-N-nitro-sourea. This shows that bacterial DNA alkylation-repair genes are able to suppress the alkylation-repair defects in human Mer- cells.
DOI：
--
发表时间：
1984
期刊：
影响因子：
--
作者：
B. Hames;J. Higgins
通讯作者：
J. Higgins