Design of protein-responsive micro-sized hydrogels for self-regulating microfluidic systems
Design of protein-responsive micro-sized hydrogels for self-regulating microfluidic systems
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DOI：
10.1088/1361-6439/aaa876
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发表时间：
2018-03-01
影响因子：
2.3
通讯作者：
Miyata, Takashi
中科院分区：
文献类型：
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作者：
Hirayama, Mayu;Tsuruta, Kazuhiro;Miyata, Takashi
已经开发了使用微全分析系统（mu-TAS）的诊断传感器，用于检测目标生物分子，例如蛋白质和唾液酸，因为它们是用于监测身体状况和疾病的信号生物分子。在这项研究中，生物分子刺激响应的微型水凝胶，表现出快速收缩响应凝集素伴刀豆球蛋白A（刀豆球蛋白A）的制备在微通道中通过光聚合使用荧光显微镜。在分子印迹技术中，以甲基丙烯酸糖氧乙酯（GEMA）为配体单体，在模板分子刀豆蛋白A（ConA）存在下，与交联剂共聚，制备了微尺寸水凝胶。ConA印迹微水凝胶显示出更大的收缩响应于目标ConA比nonimprinted微水凝胶。当缓冲溶液被切换到ConA水溶液中的Y形微通道，流速迅速变化，因为响应的收缩的微水凝胶中制备的微通道。这些结果表明，ConA印迹微水凝胶作为一个自我调节的微阀在微流控系统。
Diagnosis sensors using micro-total analysis systems (mu-TAS) have been developed for detecting target biomolecules such as proteins and saccharides because they are signal biomolecules for monitoring body conditions and diseases. In this study, biomolecularly stimuli-responsive micro-sized hydrogels that exhibited quick shrinkage in response to lectin concanavalinA (ConA) were prepared in a microchannel by photopolymerization using a fluorescence microscope. In preparing the micro-size hydrogels, glycosyloxyethyl methacrylate (GEMA) as a ligand monomer was copolymerized with a crosslinker in the presence of template ConA in molecular imprinting. The ConA-imprinted micro-hydrogel showed greater shrinkage in response to target ConA than nonimprinted micro-hydrogel. When a buffer solution was switched to an aqueous ConA solution in the Y-shaped microchannel, the flow rates changed quickly because of the responsive shrinkage of the micro-hydrogel prepared in the microchannel. These results suggest that the ConA-imprinted micro-hydrogel acted as a self-regulated microvalve in microfluidic systems.