Characterization of a filamentous biofilm community established in a cellulose-fed microbial fuel cell.
Characterization of a filamentous biofilm community established in a cellulose-fed microbial fuel cell.
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DOI：
10.1186/1471-2180-8-6
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发表时间：
2008-01-10
期刊：
影响因子：
4.2
通讯作者：
Watanabe, Kazuya
中科院分区：
文献类型：
--
作者：
Ishii, Shun'ichi;Shimoyama, Takefumi;Hotta, Yasuaki;Watanabe, Kazuya
微生物燃料电池（MFC）是利用微生物从有机物中产生电能的装置。尽管开发了高效的MFC反应器，但发电的微生物学仍有待充分理解。以稻田土壤为接种物，以纤维素为碳源和能源，构建了实验室规模的双室微生物燃料电池（MFC）。通过传代培养附着在阳极电极上的生物膜来富集发电微生物。从第一富集培养物产生0.2mA的电流，并且主要代谢物（例如，电流、甲烷和乙酸盐）在第四次富集后变得稳定。为了研究阳极生物膜中的产电微生物群落，采用电子显微镜对其进行了形态学分析，并通过16S rRNA基因克隆文库分析对群落成员进行了遗传学鉴定。电子显微镜观察发现，丝状细胞和杆状细胞与prothecae样丝状附属物大量存在于生物膜。丝状细胞和附属物通过细丝相互连接。克隆文库分析了常见的梭菌目、绿弯菌、根瘤菌目和甲烷杆菌属的细菌类型。荧光原位杂交结果表明，根瘤菌种群为杆状细胞，有丝状附属物，占总种群的30%以上。隶属于根瘤菌目的细菌构成了纤维素喂养的MFC中的主要群体，并表现出独特的形态与丝状附属物。它们被认为在降解纤维素的产电菌群中起重要作用。
Microbial fuel cells (MFCs) are devices that exploit microorganisms to generate electric power from organic matter. Despite the development of efficient MFC reactors, the microbiology of electricity generation remains to be sufficiently understood. A laboratory-scale two-chamber microbial fuel cell (MFC) was inoculated with rice paddy field soil and fed cellulose as the carbon and energy source. Electricity-generating microorganisms were enriched by subculturing biofilms that attached onto anode electrodes. An electric current of 0.2 mA was generated from the first enrichment culture, and ratios of the major metabolites (e.g., electric current, methane and acetate) became stable after the forth enrichment. In order to investigate the electrogenic microbial community in the anode biofilm, it was morphologically analyzed by electron microscopy, and community members were phylogenetically identified by 16S rRNA gene clone-library analyses. Electron microscopy revealed that filamentous cells and rod-shaped cells with prosthecae-like filamentous appendages were abundantly present in the biofilm. Filamentous cells and appendages were interconnected via thin filaments. The clone library analyses frequently detected phylotypes affiliated with Clostridiales, Chloroflexi, Rhizobiales and Methanobacterium. Fluorescence in-situ hybridization revealed that the Rhizobiales population represented rod-shaped cells with filamentous appendages and constituted over 30% of the total population. Bacteria affiliated with the Rhizobiales constituted the major population in the cellulose-fed MFC and exhibited unique morphology with filamentous appendages. They are considered to play important roles in the cellulose-degrading electrogenic community.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
3.2
作者：
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作者：
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作者：
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