Phospholipid-synthesizing enzymes in golgi membranes of the yeast, Saccharomyces cerevisiae
Phospholipid-synthesizing enzymes in golgi membranes of the yeast, Saccharomyces cerevisiae
复制标题
DOI：
10.1016/0014-5793(95)01361-x
复制
发表时间：
1995-12-18
期刊：
影响因子：
3.5
通讯作者：
Daum, G
中科院分区：
文献类型：
--
作者：
Leber, A;Hrastnik, C;Daum, G
通过类似于Zeroes等[Cell 64（1991）789-800]描述的方法分离酵母，酿酒酵母的高尔基体膜。高尔基体的标记蛋白，如Kex 2蛋白酶和GDPR，在这些制剂中高度富集。高尔基体膜的磷脂和麦角固醇含量低，磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰肌醇是该隔室的主要磷脂。高尔基体中磷脂酰丝氨酸的含量显著高于酵母体膜，含有肌醇的鞘脂，特别是肌醇磷酸神经酰胺在高尔基体膜中高度富集，在高尔基体中检测到两种磷脂合成酶，即磷脂酰肌醇合成酶和sn-1，2-二酰基甘油胆碱磷酸转移酶，其比活性超过内质网。
Golgi membranes of the yeast, Saccharomyces cerevisiae, mere isolated by a method similar to the procedure described by Cleves et al. [Cell 64 (1991) 789-800]. Marker proteins of the Golgi, such as Kex2 protease and GDPase, are highly enriched in these preparations, The phospholipid and ergosterol content of Golgi membranes is low, Phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol are the major phospholipids of this compartment. The amount of phosphatidylserine in the Golgi is significantly higher than in yeast bulk membranes, Inositol-containing sphingolipids, especially inositolphosphorylceramide, are highly enriched in Golgi membranes, Two phospholipid-synthesizing enzymes, namely phosphatidylinositol synthase and sn-1,2-diacylglycerol cholinephosphotransferase, are detected in the Golgi at a specific activity which exceeds that of the endoplasmic reticulum.