Fabrication of reconfigurable protein matrices by cracking
Fabrication of reconfigurable protein matrices by cracking
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DOI：
10.1038/nmat1365
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发表时间：
2005-05-01
期刊：
影响因子：
41.2
通讯作者：
Takayama, S
中科院分区：
文献类型：
--
作者：
Zhu, XY;Mills, KL;Takayama, S
细胞外基质和细胞之间的界面是一个动态的环境，这是至关重要的调节重要的细胞过程，如信号转导，生长，分化，运动和apoptosis.In vitrocellular研究和新的生物材料的发展将受益于矩阵，允许可逆调制的细胞粘附信号在一个规模是相称的个人粘附复合物。在这里，我们描述了制造的基板包含阵列的裂缝中，细胞粘附蛋白质被选择性地吸附。裂纹的宽度（120- 3，200 nm）在尺寸上类似于单个粘附复合物（通常为500- 3，000 nm），并且可以通过调节施加到基底的机械应变来调节。细胞的形态可以通过原位调节裂缝宽度和细胞粘附蛋白的数量来可逆地多次操纵。这些底物为评估与暴露于基质蛋白相关的细胞反应提供了新的工具。
The interface between extracellular matrices and cells is a dynamic environment that is crucial for regulating important cellular processes such as signal transduction, growth, differentiation, motility and apoptosis.In vitrocellular studies and the development of new biomaterials would benefit from matrices that allow reversible modulation of the cell adhesive signals at a scale that is commensurate with individual adhesion complexes. Here, we describe the fabrication of substrates containing arrays of cracks in which cell-adhesive proteins are selectively adsorbed. The widths of the cracks (120–3,200 nm) are similar in size to individual adhesion complexes (typically 500–3,000 nm) and can be modulated by adjusting the mechanical strain applied to the substrate. Morphology of cells can be reversibly manipulated multiple times throughin situadjustment of crack widths and hence the amount of the cell-adhesive proteins accessible to the cell. These substrates provide a new tool for assessing cellular responses associated with exposure to matrix proteins.