Proteomic analysis of platelets treated with gamma irradiation versus a commercial photochemical pathogen reduction technology
Proteomic analysis of platelets treated with gamma irradiation versus a commercial photochemical pathogen reduction technology
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DOI：
10.1111/trf.12060
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发表时间：
2013-08-01
期刊：
影响因子：
2.9
通讯作者：
Zolla, Lello
中科院分区：
文献类型：
--
作者：
Marrocco, Cristina;D'Alessandro, Angelo;Zolla, Lello
背景：目前正在测试几种策略以降低血小板（PLT）输注相关病原体传播的风险。在意大利血小板技术评估研究的框架内，我们研究了蛋白质谱的变化，用核黄素和紫外（UV）光处理后的单采血小板浓缩物（PC）的蛋白质组学（Mirasol; 6.24J/mL; 280-400nm）。对照组，伽马辐照组，在储存的第1天和第5天，通过基于凝胶的分析方法测定经Mirasol处理的单采PC（双向凝胶电泳）和质谱鉴定显著（p
BACKGROUND: Several strategies are currently being tested to reduce the risk of pathogen transmission associated with platelet (PLT) transfusion. Within the framework of the Italian Platelet Technology Assessment Study, we investigated the variations of the protein profiles (proteomics) of apheresis PLT concentrates (PCs) upon treatment with riboflavin and ultraviolet (UV) light (Mirasol; 6.24J/mL; 280-400nm).STUDY DESIGN AND METHODS: Control, gamma-irradiated, and Mirasol-treated apheresis PCs were assayed on Days 1 and 5 of storage by means of gel-based analytical approaches (two-dimensional gel electrophoresis) and mass spectrometry-based identification of significant (p