QUANTITATION OF HEPATIC GLYCOGENOLYSIS AND GLUCONEOGENESIS IN FASTING HUMANS WITH C-13 NMR
QUANTITATION OF HEPATIC GLYCOGENOLYSIS AND GLUCONEOGENESIS IN FASTING HUMANS WITH C-13 NMR
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DOI：
10.1126/science.1948033
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发表时间：
1991-10-25
期刊：
影响因子：
56.9
通讯作者：
SHULMAN, GI
中科院分区：
文献类型：
--
作者：
ROTHMAN, DL;MAGNUSSON, I;SHULMAN, GI
通过在68小时禁食期间以3- 12小时间隔连续测量肝糖原浓度，用C-13核磁共振光谱法评估人类的净肝糖原分解率。 通过从用氚化葡萄糖测量的全身葡萄糖产生速率中减去净肝糖原分解速率来计算净再生速率。在禁食的前22小时内，葡萄糖异生占总葡萄糖产量的64 +/- 5%（平均值+/-平均值的标准误差）。在随后的14小时和18小时的禁食期，葡萄糖异生分别占总葡萄糖产量的82 +/- 5%和96 +/- 1%。 这些数据表明，即使在人类禁食的前22小时内，葡萄糖生成也占总葡萄糖产量的很大一部分。
The rate of net hepatic glycogenolysis was assessed in humans by serially measuring hepatic glycogen concentration at 3- to 12-hour intervals during a 68-hour fast with C-13 nuclear magnetic resonance spectroscopy. The net rate of gluconeogenesis was calculated by subtracting the rate of net hepatic glycogenolysis from the rate of glucose production in the whole body measured with tritiated glucose. Gluconeogenesis accounted for 64 +/- 5% (mean +/- standard error of the mean) of total glucose production during the first 22 hours of fasting. In the subsequent 14-hour and 18-hour periods of the fast, gluconeogenesis accounted for 82 +/- 5% and 96 +/- 1% of total glucose production, respectively. These data show that gluconeogenesis accounts for a substantial fraction of total glucose production even during the first 22 hours of a fast in humans.