3-Phenyllactic acid production by Lactobacillus crustorum strains isolated from naturally fermented vegetables
3-Phenyllactic acid production by Lactobacillus crustorum strains isolated from naturally fermented vegetables
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DOI：
10.1016/j.lwt.2021.111780
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发表时间：
2021-09
期刊：
影响因子：
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通讯作者：
Juan Xu;Jingru Sun;Kuo-Lin Si;Chunfeng Guo
中科院分区：
文献类型：
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作者：
Juan Xu;Jingru Sun;Kuo-Lin Si;Chunfeng Guo
3-苯基乳酸是一种具有广谱抗菌和抗真菌活性的生物活性化合物，具有多种良好的药理作用。本研究对从自然发酵的中国蔬菜样品中分离到的351株乳酸菌进行了产3-聚乳酸能力的分析，从中筛选出3株产3-聚乳酸能力最强的菌株，以进一步优化发酵条件，提高其3-聚乳酸的产量。结果表明，所有菌株均能在MRS发酵液中产生3-聚乳酸，但菌株间和菌种间的3-聚乳酸产生能力存在较大差异，克氏乳杆菌的3-聚乳酸产生能力显著高于其他菌种。在优化的发酵条件下，接种于MRS发酵液中，添加60 mmol/L苯丙酮酸为底物，5.0%CaCO3为中和剂，在30℃下培养48h，菌体产量可达45.2 mmol/L 3-聚乳酸，底物转化率达73%。CRUSTORUM NWAFU 1078用于高效生产3-聚乳酸。
3-Phenyllactic acid (3-PLA) is a bioactive compound with broad-spectrum antibacterial and antifungal activity and several desirable pharmacological properties. In this study, 351 lactic acid bacterial strains isolated from naturally fermented Chinese vegetable samples were analyzed for their 3-PLA-producing ability, and three strains showing the greatest 3-PLA-producing ability were selected for further optimizing the fermentation conditions to enhance their 3-PLA yields. The results showed that all the strains were able to produce 3-PLA in MRS broth, but there were considerable variations in their 3-PLA-producing ability among the strains and among the species.Lactobacillus crustorumshowed significantly higher 3-PLA-producing ability than the other species. In particular,L. crustorumNWAFU 1078 produced up to 45.2 mmol/L 3-PLA with a 73% substrate conversion rate under the optimized fermentation conditions that the culture was inoculated into MRS broth supplemented with 60 mmol/L phenylpyruvic acid as a substrate, 5.0% CaCO3as a neutralizer and incubated for 48 h at 30 °C. This study indicates the possibility of the biotransformation of phenylpyruvic acid byL. crustorumNWAFU 1078 for highly efficient 3-PLA production.