HLA CLASS-II-RESTRICTED MYCOBACTERIUM-LEPRAE-REACTIVE T-CELL CLONES FROM LEPROSY PATIENTS ESTABLISHED WITH A MINIMAL REQUIREMENT FOR AUTOLOGOUS MONONUCLEAR-CELLS
HLA CLASS-II-RESTRICTED MYCOBACTERIUM-LEPRAE-REACTIVE T-CELL CLONES FROM LEPROSY PATIENTS ESTABLISHED WITH A MINIMAL REQUIREMENT FOR AUTOLOGOUS MONONUCLEAR-CELLS
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DOI：
10.1111/j.1365-3083.1986.tb01947.x
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发表时间：
1986-01-01
影响因子：
3.7
通讯作者：
DEVRIES, RRP
中科院分区：
文献类型：
--
作者：
HAANEN, JBAG;OTTENHOFF, THM;DEVRIES, RRP
本文报道了一种用EB病毒转化的自体B细胞作为抗原提呈细胞克隆麻风分枝杆菌反应性T淋巴细胞的有效方法。这种方法的两个优点是，它大大减少了获得和繁殖这些T细胞克隆（TLC）所需的自体外周血单核细胞（< 107个细胞）的数量，并且使我们能够将单个TLC扩增到大量细胞（> 108个细胞）。这样就绕过了克隆T淋巴细胞的主要障碍，这对病人来说尤其重要。到目前为止，已经建立了三名麻风病患者的TLC。这些TLC是HLA II类限制性的M。麻风导向反应当针对许多其他分枝杆菌抗原进行测试时，在进一步扩展几种显著不同的抗原识别模式后观察到抗原反应性的显著增强;迄今为止，一种TLC仅识别M。麻风抗原
This report describes an effective method for the cloning of Mycobacterium leprae-reactive T lymphocytes with Epstein-Barr-virus transformed autologous B cells as antigen-presenting cells. The two advantages of this method are that it drastically reduces the number of autologous peripheral blood mononuclear cells (< 107 cells) needed to obtain and propagate these T-cell clones (TLC), and that enables us to expand individual TLC to large numbers of cells (> 108). Thus the major obstacles for the cloning of T lymphocytes-especially important with regard to patients-are bypassed. Thus far, TLC from three leprosy patients have been established. These TLC are HLA class II restricted in their M. leprae-directed response. A marked enhancement in antigen responsiveness was observed after further expansion of several strikingly different antigen recognition patterns when tested against a number of other mycobacterial antigens; one TLC so far recognizes only M. leprae antigens.