IDENTIFICATION OF BEAUVERIA-BASSIANA EXTRACELLULAR PROTEASE AS A VIRULENCE FACTOR IN PATHOGENICITY TOWARD THE MIGRATORY GRASSHOPPER, MELANOPLUS-SANGUINIPES
IDENTIFICATION OF BEAUVERIA-BASSIANA EXTRACELLULAR PROTEASE AS A VIRULENCE FACTOR IN PATHOGENICITY TOWARD THE MIGRATORY GRASSHOPPER, MELANOPLUS-SANGUINIPES
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DOI：
10.1016/0022-2011(90)90123-n
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发表时间：
1990-11-01
影响因子：
3.4
通讯作者：
KHACHATOURIANS, GG
中科院分区：
文献类型：
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作者：
BIDOCHKA, MJ;KHACHATOURIANS, GG
球孢白僵菌胞外蛋白酶能水解血足黑蝗角质层中的蛋白质。B的蛋白酶活性菌株和蛋白酶缺陷菌株。在液体培养条件下测定了白僵菌胞外蛋白酶的产生量，并测定了它们对M.血足动物。一种紫外辐射衍生的蛋白酶-精通菌株，当生长在1%（w/v）明胶液体培养基或1%（w/v）研磨M.血足类角质层液体培养基。蛋白酶活性菌株对迁飞性蝗虫的LT 50最低（5.71-6.80天），而蛋白酶缺陷菌株的LT 50最高（11.34天）。各种菌株在液体培养物中生长2天后的蛋白酶产量与其相关的LT 50之间呈负相关。我们的结论是，在这项研究中，蛋白酶生产的B。蛋白酶缺陷型菌株的致病力较低，延长了对M.血足动物。
Beauveria bassiana extracellular protease was shown to hydrolyze protein in cuticle of the migratory grasshopper, Melanoplus sanguinipes. Protease-proficient and protease-deficient strains of B. bassiana were assessed for production of extracellular protease in liquid culture and their virulence, as measured by LT50, toward M. sanguinipes. A uv irradiation-derived protease-proficient strains when grown in 1% (w/v) gelatin liquid medium or 1% (w/v) ground M. sanguinipes cuticle liquid medium. The protease-proficient strains showed the lowest LT50 (5.71-6.80 days) against the migratory grasshopper, while the protease-deficient strain had the highest LT50 (11.34 days). There was a negative relationship between protease production after 2 days of growth in liquid culture by the various strains and their associated LT50. We conclude that, in this study, protease production by the B. bassiana protease-deficient strain was low enough so that it extended the time course of pathogenesis toward M. sanguinipes.