Hyperthermia influences fate determination of neural stem cells with lncRNAs alterations in the early differentiation.
Hyperthermia influences fate determination of neural stem cells with lncRNAs alterations in the early differentiation.
复制标题
高温通过早期分化中 lncRNA 的改变影响神经干细胞的命运决定。
DOI：
10.1371/journal.pone.0171359
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Wang L;Deng Y;Duan D;Sun S;Ge L;Zhuo Y;Yuan T;Wu P;Wang H;Lu M;Xia Y
温度是神经干细胞（NSC）微环境中的一个重要参数，然而，关于高温对NSC命运决定的精确影响或长非编码（lnc）RNA在此过程中的作用知之甚少。本研究使用在两种不同温度下培养的神经干细胞解决了这一问题。将神经干细胞分为37和40个NSC组，分别在37°C和40°C下培养72 h。通过流式细胞术和蛋白质印迹法评估神经元或神经胶质细胞分化。实时荧光定量PCR检测LncRNA表达。神经元标志物Tuj-1和胶质细胞标志物胶质细胞酸性蛋白阳性的细胞数量在40 NSC组中高于37 NSC组。两组也显示出不同的lncRNA表达谱。高温促进神经干细胞的神经元和胶质细胞分化，这涉及到特定的lncRNA。
Temperature is an important parameter in the microenvironment of neural stem cells (NSCs); however, little is known about the precise effects of hyperthermia on fate determination in NSCs or the role of long non-coding (lnc)RNAs in this process. This was addressed in the present study using NSCs cultured at two different temperatures. NSCs were divided into 37NSC and 40NSC groups that were cultured at 37°C or 40°C, respectively, for 72 h. Neuronal or glial cell differentiation was evaluated by flow cytometry and western blotting. LncRNA expression was detected by quantitative real-time PCR. The numbers of cells positive for the neuronal marker Tuj-1 and the glial cell marker glial fibrillary acidic protein were higher in the 40NSC than in the 37NSC group. The two groups also showed distinct lncRNA expression profiles. Hyperthermia promotes neuronal and glial differentiation in NSCs, which involves specific lncRNAs.