Non-colony type monolayer culture of human embryonic stem cells.
Non-colony type monolayer culture of human embryonic stem cells.
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DOI：
10.1016/j.scr.2012.06.003
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发表时间：
2012-11
影响因子：
1.2
通讯作者：
McKay, Ronald D. G.
中科院分区：
文献类型：
--
作者：
Chen, Kevin G.;Mallon, Barbara S.;Hamilton, Rebecca S.;Kozhich, Olga A.;Park, Kyeyoon;Hoeppner, Daniel J.;Robey, Pamela G.;McKay, Ronald D. G.
以人类胚胎干细胞（hESC）技术为依托的再生医学为许多严重疾病的治疗开辟了有希望的新途径。然而，由于体外hESC生长的难治性，这种方法受到所需细胞的有限生产的限制。在当前的培养条件下，细胞应激、生长速率和异质细胞状态的控制不足进一步阻碍了它的发展。在这项研究中，我们报告了一种新的基于非集落型单层（NCM）生长的细胞培养方法。经畸胎瘤实验证实，NCM下的人类ESCs仍具有多能性，并具有分化为三个胚层的潜力。这种NCM培养已被证明可以均匀化细胞状态，精确控制生长速率，显著增加细胞产量，并在不损害染色体完整性的情况下从低温保存中提高hESC的恢复。该培养系统简单、健壮、可扩展，适合于高通量筛选和药物发现。
Regenerative medicine, relying on human embryonic stem cell (hESC) technology, opens promising new avenues for therapy of many severe diseases. However, this approach is restricted by limited production of the desired cells due to the refractory properties of hESC growth in vitro. It is further hindered by insufficient control of cellular stress, growth rates, and heterogeneous cellular states under current culture conditions. In this study, we report a novel cell culture method based on a non-colony type monolayer (NCM) growth. Human ESCs under NCM remain pluripotent as determined by teratoma assays and sustain the potential to differentiate into three germ layers. This NCM culture has been shown to homogenize cellular states, precisely control growth rates, significantly increase cell production, and enhance hESC recovery from cryopreservation without compromising chromosomal integrity. This culture system is simple, robust, scalable, and suitable for high-throughput screening and drug discovery.
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影响因子：
3.7
作者：
Emre N;Vidal JG;Elia J;O'Connor ED;Paramban RI;Hefferan MP;Navarro R;Goldberg DS;Varki NM;Marsala M;Carson CT
通讯作者：
Carson CT
影响因子：
46.9
作者：
Hockemeyer, Dirk;Soldner, Frank;Beard, Caroline;Gao, Qing;Mitalipova, Maisam;DeKelver, Russell C.;Katibah, George E.;Amora, Ranier;Boydston, Elizabeth A.;Zeitler, Bryan;Meng, Xiangdong;Miller, Jeffrey C.;Zhang, Lei;Rebar, Edward J.;Gregory, Philip D.;Urnov, Fyodor D.;Jaenisch, Rudolf
通讯作者：
Jaenisch, Rudolf
影响因子：
4.8
作者：
Meisner, Lorraine Faxon;Johnson, Julie A.
通讯作者：
Johnson, Julie A.
影响因子：
4
作者：
Krawetz, Roman J.;Li, Xiangyun;Rancourt, Derrick E.
通讯作者：
Rancourt, Derrick E.
影响因子：
48
作者：
Braam, Stefan R.;Denning, Chris;Mummery, Christine L.
通讯作者：
Mummery, Christine L.