KLONER; a computer program to simulate recombinant DNA strategies by restriction map manipulation
KLONER; a computer program to simulate recombinant DNA strategies by restriction map manipulation
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DOI：
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发表时间：
1984
期刊：
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通讯作者：
P. Caron
中科院分区：
文献类型：
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作者：
P. Caron
描述了一种计算机程序，该程序允许操纵各种DNA片段的限制性图谱，以演示用于DNA克隆的技术，并预测和/或确认实验结果。该程序能够阅读几种不同目的DNA分子的限制酶切割位点。然后编辑这些信息以形成限制性图谱，然后可以通过用限制性内切酶消化和用其他常见的DNA修饰酶处理来处理。然后可以通过以所有可能的方向连接具有互补末端的片段来模拟连接，产生产物的限制性图谱。然后可以通过用适当的限制性内切核酸酶进行物理作图来进一步分析所得重组体。这个程序是在Apple II计算机上用Pascal语言编写的。
A computer program is described which allows for the manipulation of restriction maps of various DNA fragments to demonstrate techniques used in DNA cloning and to predict and/or confirm experimental results. This program is capable of reading in restriction enzyme cleavage sites for several different DNA molecules of interest. This information is then compiled in order to form restriction maps which can then be processed by digestion with restriction endonucleases and treatment with other common DNA modifying enzymes. Ligation can then be simulated by joining fragments with complementary ends in all possible orientations, producing restriction maps of the products. The resulting recombinants can then be further analyzed by physical mapping with appropriate restriction endonucleases. This program was written in Pascal on an Apple II computer.