SPECTRAL FUNDUS AUTOFLUORESCENCE EXCITATION AND EMISSION IN ABCA4-RELATED RETINOPATHY
SPECTRAL FUNDUS AUTOFLUORESCENCE EXCITATION AND EMISSION IN ABCA4-RELATED RETINOPATHY
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DOI：
10.1097/iae.0000000000002726
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发表时间：
2020-12-01
影响因子：
3.3
通讯作者：
Herrmann, Philipp
中科院分区：
文献类型：
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作者：
Muller, Philipp L.;Dysli, Chantal;Herrmann, Philipp
目的：目的：系统、纵向研究ABCA 4相关视网膜病变（ABCA 4-related retinopathy）患者在不同眼底自发荧光（autofluorescence，AF）激发和发射光谱下的荧光特征。通过共焦扫描激光检眼镜获得的蓝色（BAF）、绿色（GAF）和近红外（NIR-AF）激发波长的自体荧光成像与通过创新的基于共焦发光二极管的视网膜成像系统获得的AF成像进行了比较（彩色AF），允许分离短（绿色发射荧光组分）和长结果：总体上，Color-AF、BAF和GAF揭示了类似的超自发荧光荧光的呈现。在NIR-AF中，主要显示红色发射荧光成分的斑点与超自发荧光闪烁相匹配。在5至14个月的观察时间内，闪烁显示AF发射光谱向较短波长的过渡（红色发射荧光成分向绿色发射荧光成分），与光学相干断层扫描中重叠的视网膜外带的进展性破坏相关。较新的hyperautofluorescent flections通常显示主要是红色发射fluorescentcomponent.Conclusion：通过分离的AF光谱，重构的荧光团和相关的结构变化可以随着时间的推移进行监测，表明一种新的和敏感的替代标记物的疾病进展和潜在的治疗效果。
Purpose: To systematically and longitudinally investigate the characteristics of flecks in ABCA4-related retinopathy under different fundus autofluorescence (AF) excitation and emission spectra.Methods: A total of 132 eyes of 66 patients with ABCA4-related retinopathy were investigated using multimodal AF imaging and spectral domain optical coherence tomography. Autofluorescence imaging with blue (BAF), green (GAF), and near-infrared (NIR-AF) excitation wavelengths obtained by a confocal scanning laser ophthalmoscope was compared with AF imaging obtained by an innovative confocal light-emitting diode-based retinal imaging system (Color-AF) that allows for separation of short (green emission fluorescent component) and long (red emission fluorescent component) autofluorescence emission components.Results: Color-AF, BAF, and GAF, overall, revealed similar presentation of hyperautofluorescent flecks. Flecks that showed predominantly red emission fluorescent component matched with hyperautofluorescent flecks in NIR-AF. Over the observation time of 5 to 14 months, flecks showed a transition in the AF emission spectrum to shorter wavelengths (red emission fluorescent component to green emission fluorescent component), associated with a progressed disruption of overlaying outer retinal bands in optical coherence tomography. Newer hyperautofluorescent flecks usually revealed predominantly red emission fluorescent component.Conclusion: By separation of the AF spectra, the remodeling of fluorophores and associated structural changes can be monitored over time indicating a novel and susceptible surrogate marker for disease progression and potential therapeutic effects.