Spatial and temporally restricted expression of chemokines and chemokine receptors in the developing human kidney
Spatial and temporally restricted expression of chemokines and chemokine receptors in the developing human kidney
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DOI：
10.1681/asn.v134957
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发表时间：
2002-04-01
影响因子：
13.6
通讯作者：
Nelson, PJ
中科院分区：
文献类型：
--
作者：
Gröne, HJ;Cohen, CD;Nelson, PJ
细胞的定向迁移、细胞间的粘附和增殖控制是后肾发育过程中的关键事件。趋化因子是一类选择性控制细胞迁移、活化、增殖和粘附的蛋白。通过免疫组织化学分析和激光显微解剖后肾组织的实时逆转录pcr检测，研究了一系列趋化因子和趋化因子受体在人肾发育过程中的表达。结果表明肾源性母细胞内的单核细胞样细胞局部表达干扰素诱导蛋白-10/ cxcl10，一种CXCR3的配体。分散在发育器官中的单核样细胞表达CX(3)CR1。基质细胞衍生因子-1/CXCL12受体CXCR4的表达也仅限于基质cd34阳性细胞。相比之下，基质细胞衍生因子-1/ CXCL12、fractalkine和CXCR3的表达首先在逗号或s形体阶段被观察到。在毛细血管袢期表达强度增强，主要在肾小球系膜柄和内皮细胞中表达。这些蛋白可能在肾脏发育中起调节作用。因为在个体发育过程中表达的基因通常在组织再生中发挥作用，这些胚胎趋化因子/趋化因子受体模式可能在肾脏损伤和修复中很重要。
The directed migration of cells, cell-cell adhesion, and the control of proliferation are key events during metanephric development. The chemokines are a family of proteins that selectively control aspects of cell migration, activation, proliferation, and adhesion. The expression of a series of chemokines and chemokine receptors during human renal development was investigated by using immunohistochemical analyses and real-time reverse transcription-PCR assays of defined laser-microdissected metanephric structures. The results demonstrate that mononuclear cell-like cells within the nephrogenic blastema focally express interferon-inducible protein-10/CXCL 10, a ligand for CXCR3. Mononuclear-like cells dispersed through the developing organ express CX(3)CR1. Expression of CXCR4, the receptor for stromal cell-derived factor-1/CXCL12, is also limited to stromal CD34-positive cells. In contrast, the expression of stromal cell-derived factor-1/ CXCL12, fractalkine, and CXCR3 is first observed in the comma- or S-shaped body stage. The intensity of this expression becomes stronger in the capillary loop stage, and expression is mainly observed in the mesangial stalk and endothelial cells of the glomeruli. These proteins may play modulatory roles in kidney development. Because genes that are expressed during ontogeny often play a role in tissue regeneration, these embryonal chemokine/chemokine receptor patterns may be important in renal injury and repair.