Effect of antibodies against seminal vesicle secretion on fertility in the rat
Effect of antibodies against seminal vesicle secretion on fertility in the rat
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DOI：
10.1017/s096719949900060x
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发表时间：
1999-08-01
期刊：
影响因子：
1.7
通讯作者：
Esponda, P
中科院分区：
文献类型：
--
作者：
Carballada, R;Esponda, P
用大鼠和小鼠精囊分泌物（SVS）对可育雌性Wistar大鼠进行免疫，以检测同种抗体的产生和抗体对生育力的影响。26%的大鼠和小鼠SVS免疫雌性动物在治疗后不育。通过ELISA滴定血清并用于蛋白质印迹以检测识别的蛋白质。虽然无论是抗体滴度，也不是由血清识别的蛋白质表现出密切的关系与生育力的程度，在所有女性的最高抗体滴度的液体从生殖道中发现在输卵管液和发情的夜晚。这一事实表明抗体的作用部位可能是输卵管。使用小鼠SVS作为免疫原获得了类似的结果-这一事实可能与两个物种的SVS之间的抗原相似性有关。抗体与精子反应，但不与卵子或胚胎反应。对从不育雌性收集的胚胎进行的分析表明，受精和正常胚胎发生延迟。我们的研究结果表明，SVS蛋白是抗原性的，这些抗原与精子结合，并可能参与早期受精前事件，如获能或精子运输。
Fertile female Wistar rats were immunised against rat and mouse seminal vesicle secretion (SVS) to test the production of allo-antibodies and the effect of the antibodies elicited on fertility. Twenty-six per cent of the rat and mouse SVS-immunised females were infertile after the treatment. The sera were titrated by ELISA and used in Western blots to detect the proteins recognised. Although neither the antibody titres nor the proteins recognised by the sera showed a close relation with the degree of fertility, in all females the highest antibody titre in the fluids from the genital tract was found in the oviductal fluid and during the night of oestrus. This fact suggested that the site of action of the antibody could be the oviduct. Similar results were obtained using mouse SVS as immunogen - a fact that can be related to the antigenic similarity between the SVS of the two species. The antibodies react with the spermatozoa but not with eggs or embryos. Analyses performed on embryos collected from sterile females showed that there was a delay in fertilisation and normal embryogenesis. Our results suggest that SVS proteins are antigenic and that these antigens are bound to the spermatozoa and could take part in ear ly pre-fertilisation events such as capacitation or sperm transport.