Dimensionally stable and bioactive membrane for guided bone regeneration: An in vitro study.
Dimensionally stable and bioactive membrane for guided bone regeneration: An in vitro study.
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DOI：
10.1002/jbm.b.33430
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发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Bottino MC
中科院分区：
文献类型：
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作者：
Rowe MJ;Kamocki K;Pankajakshan D;Li D;Bruzzaniti A;Thomas V;Blanchard SB;Bottino MC
基于聚（DL-丙交酯）（PLA）和聚（ε-己内酯）（PCL）的复合纤维电纺膜被设计为包含硼酸盐生物活性玻璃（BBG），用于引导骨再生（GBR）的潜在目的。使用扫描和透射电子显微镜对纤维进行了表征，分别证实了膜的亚微米纤维排列和 BBG 颗粒的成功掺入。使用缝线拉拔测试评估膜的选定机械性能。添加 10 wt.% 的 BBG 会产生类似的刚度，但更重要的是，与水合条件下市售的 Epiguide® (1.06±0.24 N*mm) 相比，它会产生明显更强的膜 (2.37±0.51 N*mm)。在磷酸盐缓冲溶液中孵育 24 小时至 9 天后测定稳定性（收缩）。掺入或不掺入 BBG 的 PLA:PCL 膜的尺寸稳定性 (10.07-16.08%) 与 Epiguide® (14.28%) 相似。细胞增殖测定表明，体外培养 7 天后，含 BBG 的膜（6.4 倍）的前成骨细胞增殖率高于不含 BBG 的膜（4-5.8 倍）和 EpiGuide®（4.5 倍）。总的来说，我们的结果证明了通过静电纺丝合成稳定的、基于聚合物的亚微米纤维 BBG 膜的能力，该膜能够维持成骨细胞的附着和增殖——这是引导骨再生的一个有前途的属性。
Composite fibrous electrospun membranes based on poly(DL-lactide) (PLA) and poly(ε-caprolactone) (PCL) were engineered to include borate bioactive glass (BBG) for the potential purposes of guided bone regeneration (GBR). The fibers were characterized using scanning and transmission electron microscopies, which respectively confirmed the submicron fibrous arrangement of the membranes and the successful incorporation of BBG particles. Selected mechanical properties of the membranes were evaluated using the suture pullout test. The addition of BBG at 10 wt.% led to similar stiffness, but more importantly, it led to a significantly stronger (2.37±0.51 N*mm) membrane when compared to the commercially available Epiguide® (1.06±0.24 N*mm) under hydrated conditions. Stability (shrinkage) was determined after incubation in a phosphate buffer solution from 24 h up to 9 days. The dimensional stability of the PLA:PCL-based membranes with or without BBG incorporation (10.07-16.08%) was similar to that of Epiguide® (14.28%). Cell proliferation assays demonstrated a higher rate of pre-osteoblasts proliferation on BBG-containing membranes (6.4-fold) over BBG-free membranes (4-5.8-fold) and EpiGuide® (4.5-fold), following 7 days of in vitro culture. Collectively, our results demonstrated the ability to synthesize, via electrospinning, stable, polymer-based submicron fibrous BBG-containing membranes capable of sustaining osteoblastic attachment and proliferation—a promising attribute in guided bone regeneration.