Influence of arbuscular mycorrhiza on the growth and antioxidative activity in cyclamen under heat stress
Influence of arbuscular mycorrhiza on the growth and antioxidative activity in cyclamen under heat stress
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DOI：
10.1007/s00572-013-0477-z
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发表时间：
2013-07-01
期刊：
影响因子：
3.9
通讯作者：
Matsubara, Yoh-ichi
中科院分区：
文献类型：
--
作者：
Maya, Moslama Aktar;Matsubara, Yoh-ichi
研究了丛枝菌根 (AM) 真菌 Glomus fasciculatum 对仙客来 (Cyclamen persicum Mill.) 植物生长、热应激反应和抗氧化活性的影响。将仙客来植物（接种或未接种 AM 真菌）置于 17-20°C 的商业盆栽介质中，在温室中培养 12 周，随后在生长室中经受两种温度条件。最初，植物在 20°C 的无热应激 (HS-) 条件下生长 4 周，然后在 30°C 的热应激 (HS+) 条件下再生长 4 周。比较了 G. fasciculatum 接种和未接种植物之间不同的形态和生理生长参数。与对照相比，菌根共生显着增强了植物的生物量产生和 HS + 反应。在对照植物中观察到严重的叶子褐变率(80-100%)，而菌根植物在HS+条件下表现出最小的叶子褐变率。菌根植物表现出超氧化物歧化酶和抗坏血酸过氧化物酶等抗氧化酶活性的增加，以及抗坏血酸和多酚含量的增加。在每种温度条件下，菌根植物中的 2,2-二苯基-1-三硝基苯肼自由基清除活性也表现出比对照植物更大的反应。结果表明，在仙客来植物中，AM真菌定殖通过增加抗氧化活性减轻热应激损害，并且菌根共生强烈增强温度胁迫耐受性，促进植物生长并增加热胁迫下宿主生物量。
The influence of the arbuscular mycorrhizal (AM) fungus, Glomus fasciculatum, on the growth, heat stress responses and the antioxidative activity in cyclamen (Cyclamen persicum Mill.) plants was studied. Cyclamen plants (inoculated or not with the AM fungus) were placed in a commercial potting media at 17-20 A degrees C for 12 weeks in a greenhouse and subsequently subjected to two temperature conditions in a growth chamber. Initially, plants were grown at 20 A degrees C for 4 weeks as a no heat stress (HS-) condition, followed by 30 A degrees C for another 4 weeks as a heat stress (HS+) condition. Different morphological and physiological growth parameters were compared between G. fasciculatum-inoculated and noninoculated plants. The mycorrhizal symbiosis markedly enhanced biomass production and HS + responses in plants compared to that in the controls. A severe rate of leaf browning (80-100 %) was observed in control plants, whereas the mycorrhizal plants showed a minimum rate of leaf browning under HS + conditions. The mycorrhizal plants showed an increase activity of antioxidative enzymes such as superoxide dismutase and ascorbate peroxidase, as well as an increase in ascorbic acid and polyphenol contents. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity also showed a greater response in mycorrhizal plants than in the control plants under each temperature condition. The results indicate that in cyclamen plants, AM fungal colonisation alleviated heat stress damage through an increased antioxidative activity and that the mycorrhizal symbiosis strongly enhanced temperature stress tolerance which promoted plant growth and increased the host biomass under heat stress.