Low Prevalence of Drug Resistance Transmitted Virus in HIV Type 1-Infected ARV-Naive Patients in Cambodia
Low Prevalence of Drug Resistance Transmitted Virus in HIV Type 1-Infected ARV-Naive Patients in Cambodia
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DOI：
10.1089/aid.2008.0305
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发表时间：
2009-05-01
影响因子：
1.5
通讯作者：
Eric, Nerrienet
中科院分区：
文献类型：
--
作者：
Janin, Nouhin;Sopheak, Ngin;Eric, Nerrienet
在2006年11月至2007年6月期间，对67名未接受过抗逆转录病毒治疗的柬埔寨患者的HIV-1逆转录酶（RT）和蛋白酶（PR）基因进行扩增和测序。入选时，HIV感染的中位年龄和持续时间分别为28岁和1.1年。中位CD 4和HIV-1 RNA为611个细胞/ml [IQR：525-759]和4.0 log（10）拷贝/ml [IQR：3.4-4.6]。在67株HIV-1中，95.5%为CRF 01_AE病毒（n = 64），而3株与B亚型聚簇。RT分析表明，67例患者中仅1例存在K103 N和M184 V突变，对NVP/EFV和3 TC/FTC耐药。在59个扩增的蛋白酶基因中未检测到对蛋白酶抑制剂的原发耐药。在我们的研究中，未接受过药物治疗的患者中传播的耐药菌株的患病率为1.49%（IC 95%：0.04-8.04%），这是很低的。应定期进行耐药传播病毒调查，以增加柬埔寨获得高效抗逆转录病毒疗法的机会。
Between November 2006 and June 2007, HIV-1 reverse transcriptase (RT) and protease (PR) genes of 67 ARV-naive Cambodian patients were amplified and sequenced. At inclusion, the median age and duration of HIV infection were 28 and 1.1 years, respectively. The median CD4 and HIV-1 RNA were 611 cells/ml [IQR: 525-759] and 4.0 log(10) copies/ml [IQR: 3.4-4.6]. Among 67 HIV-1 strains, 95.5% were CRF 01_AE viruses (n = 64) whereas three clustered with subtype B. RT analysis indicated that only 1 patient out of 67, presenting K103N and M184V mutations, was resistant to NVP/EFV and 3TC/FTC. No primary resistance to protease inhibitors was detected in 59 amplified protease genes. The 1.49% (IC 95%: 0.04-8.04%) prevalence of transmitted drug-resistant strains in drug-naive patients was low in our study. Surveys of drug-resistant transmitted viruses should be regularly performed regarding the increasing access to HAART in Cambodia.