IMPROVED STABILITY OF GENETIC SEX-SEPARATION STRAINS FOR THE MEDITERRANEAN FRUIT-FLY, CERATITIS-CAPITATA
IMPROVED STABILITY OF GENETIC SEX-SEPARATION STRAINS FOR THE MEDITERRANEAN FRUIT-FLY, CERATITIS-CAPITATA
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DOI：
10.1139/g94-009
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发表时间：
1994-02-01
期刊：
影响因子：
3.1
通讯作者：
KERREMANS, P
中科院分区：
文献类型：
--
作者：
FRANZ, G;GENCHEVA, E;KERREMANS, P
在地中海实蝇（Ceratitis capitata）现有的遗传性别鉴定菌株中，雄性重组导致在大量饲养条件下性别鉴定机制的崩溃。分解的速率取决于重组频率和重组体的适合度。我们测试了两种不同的性别鉴定基因，白色蛹和温度敏感致死基因，结合易位T（Y;5）30 C。两种性别鉴定菌株都被分解了，尽管速度非常不同。在白色蛹菌株的情况下，在培养菌株15代后观察到3.5%的重组体。第二个菌株利用白色蛹和温度敏感致死性作为选择标记，经过6代后已经达到相当的水平，并在第9代完全分解。在这些菌株中，由于T（Y;5）30 C断裂点和性别鉴定基因相距较远，重组频率较高。为了补救这种情况，我们已经分离出四个新的易位与断点位于更接近性别决定基因。用基于这些易位的菌株模拟大量饲养几代，在所用条件下未观察到分解。
In the existing genetic sexing strains for the medfly, Ceratitis capitata, male recombination leads to breakdown of the sexing mechanism under mass rearing conditions. The rate of breakdown depends on the recombination frequency and on the fitness of the recombinants. We have tested two different sexing genes, white pupa and a temperature sensitive lethal, in combination with the translocation T(Y;5)30C. Both sexing strains broke down, although at very different rates. In the case of the white pupa strain, 3.5% recombinants were observed after rearing the strain for 15 generations. The second strain, utilizing white pupa and the temperature sensitive lethal as selectable markers, already reached a comparable level after six generations and was broken down completely in the ninth generation. In these strains the frequency of recombination is high because the breakpoint of T(Y;5)30C and the sexing gene(s) are far apart. To remedy the situation, we have isolated four new translocations with breakpoints located closer to the sexing genes. Mass rearing was simulated for several generations with strains based on these translocations and no breakdown was observed under the conditions used.