Glutamate synthase 1 is involved in iron-deficiency response and long-distance transportation inArabidopsis
Glutamate synthase 1 is involved in iron-deficiency response and long-distance transportation inArabidopsis
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谷氨酸合酶1参与拟南芥缺铁反应和长距离运输
DOI：
10.1111/jipb.12985
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发表时间：
2020-07-20
影响因子：
11.4
通讯作者：
Wu, Huilan
中科院分区：
文献类型：
--
作者：
Cui, Man;Gu, Mengjun;Wu, Huilan
铁是植物生长所必需的微量元素。从土壤中吸收后，铁被配体螯合并从根转移到芽中供随后利用。然而，参与铁螯合的配体的数量尚不清楚。在这项研究中，我们确定并证明了GLU1，它编码铁氧还蛋白依赖性谷氨酸合酶，参与铁稳态。首先，缺铁条件下GLU 1的表达被强烈诱导。其次，GLU 1的损伤导致根和茎中许多缺铁反应基因的转录降低。与野生型相比，突变体植株地上部铁浓度降低，在铁限制条件下表现出严重的叶片失绿。第三，GLU 1的产物谷氨酸能螯合铁离子，促进铁的转运。最后，我们还发现在培养基中添加谷氨酸可以减轻镉对植物的毒害。总之，我们的研究结果提供了证据表明，GLU1参与铁稳态通过影响谷氨酸合成缺铁条件下在拟南芥。
Iron is an essential microelement for plant growth. After uptake from the soil, iron is chelated by ligands and translocated from roots to shoots for subsequent utilization. However, the number of ligands involved in iron chelation is unclear. In this study, we identified and demonstrated that GLU1, which encodes a ferredoxin-dependent glutamate synthase, was involved in iron homeostasis. First, the expression ofGLU1was strongly induced by iron deficiency condition. Second, lesion ofGLU1results in reduced transcription of many iron-deficiency-responsive genes in roots and shoots. The mutant plants revealed a decreased iron concentration in the shoots, and displayed severe leaf chlorosis under the condition of Fe limitation, compared to wild-type. Third, the product of GLU1, glutamate, could chelate ironin vivoand promote iron transportation. Last, we also found that supplementation of glutamate in the medium can alleviate cadmium toxicity in plants. Overall, our results provide evidence that GLU1 is involved in iron homeostasis through affecting glutamate synthesis under iron deficiency conditions inArabidopsis.