Impact of borderline-subclinical hypothyroidism on subsequent pregnancy outcome in women with unexplained recurrent pregnancy loss
Impact of borderline-subclinical hypothyroidism on subsequent pregnancy outcome in women with unexplained recurrent pregnancy loss
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边缘性亚临床甲状腺功能减退症对不明原因反复流产妇女随后妊娠结局的影响
DOI：
10.1111/jog.13319
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Tanaka M
中科院分区：
文献类型：
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作者：
Uchida S;Maruyama T;Kagami M;Miki F;Hihara H;Katakura S;Yoshimasa Y;Masuda H;Uchida H;Tanaka M
子宫内膜异位症虽然是一种良性疾病，但它与癌症有一些共同的特征，如侵袭性和转移的可能性。本研究旨在探讨人类子宫内膜异位症lesions.Material和methodsThirteen子宫内膜异位症患者和10名对照妇女子宫内膜异位症进行手术良性适应症的上皮间质转化状态。我们通过免疫组织化学检测了E-钙粘蛋白、波形蛋白和上皮-间质转化诱导的转录因子（如Snail和ZEB 1）的表达。我们评估了每种标记物在卵巢上皮性病变（卵巢上皮性瘤、深部浸润性子宫内膜异位症、子宫腺肌病）和正常子宫内膜上皮细胞中的表达。结果免疫组化分析显示，正常乳腺和乳腺增生病组织中均有E‐cadherin、vimentin和Snail表达，但仅乳腺增生病组织中有ZEB 1表达。此外，ZEB 1最常在浸润性子宫内膜异位症的上皮细胞中观察到。血清CA 125水平高的子宫内膜异位症患者更容易有ZEB 1阳性病灶。ZEB 1在子宫内膜异位病灶的上皮细胞中的优先表达表明，这些细胞可能至少部分地具有比正常子宫内膜更高水平的间充质特征，这可能是通过ZEB 1驱动的上皮-间充质转化，并且ZEB 1可以成为子宫内膜异位症侵袭性或严重程度的潜在指标。
IntroductionAlthough endometriosis is a benign disease, it shares some features with cancers, such as invasiveness and the potential to metastasize. This study sought to investigate the epithelial‐mesenchymal transition status in human endometriotic lesions.Material and methodsThirteen endometriosis patients and 10 control women without endometriosis undergoing surgery for benign indications were recruited. We examined the expression of E‐cadherin, vimentin, and epithelial‐mesenchymal transition‐induced transcriptional factors, such as Snail and ZEB1, by immunohistochemistry. We evaluated the expression of each marker in epithelial cells of both endometriotic lesions (ovarian endometrioma, deep infiltrating endometriosis, adenomyosis) and normal endometria. The correlation between ZEB1 expression and serum level of CA125 was also investigated.ResultsImmunohistochemical analysis revealed that although E‐cadherin, vimentin, and Snail were expressed in epithelia of normal endometria and endometriotic lesions, ZEB1 expression was only expressed in epithelia of endometriotic lesions. Additionally, ZEB1 was most frequently observed in epithelial cells of invasive endometriosis. The endometriosis patients with high serum CA125 level were more likely to have ZEB1‐positive lesions.ConclusionsThis is the first observation of ZEB1 expression in epithelial cells of benign disease. The preferential expression of ZEB1 in epithelial cells of endometriotic lesions suggests that these cells may have, at least in part, a higher level of mesenchymal features possibly via ZEB1‐driven epithelial‐mesenchymal transition than normal endometria and that ZEB1 can be a potential indicator of invasiveness or severity of endometriosis.