Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid
Fed-batch fermentation of Ganoderma lucidum for hyperproduction of polysaccharide and ganoderic acid
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DOI：
10.1016/s0141-0229(02)00066-2
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发表时间：
2002-07-01
影响因子：
3.4
通讯作者：
Zhong, JJ
中科院分区：
文献类型：
--
作者：
Tang, YJ;Zhong, JJ
研究了灵芝补料分批发酵同时高效生产灵芝酸和灵芝多糖的工艺。首先研究了碳源和初始糖浓度对赤霉素和多糖产量的影响。以蔗糖为碳源有利于胞外多糖（EPS）的产生，但细胞生长不佳。乳糖有利于细胞生长、GA和胞内多糖的产生。当初始乳糖浓度超过35 g/l时，GA积累减少。当乳糖残留浓度在10 ~ 5g/l时，脉冲补料可显著提高赤霉素的产量。摇瓶补料分批发酵过程可以成功地再现在搅拌生物反应器（STR）与溶解氧（DO）控制在20和35%的空气饱和度在发酵过程中。STR和烧瓶中的最大细胞密度分别达到21.89 +/- 0.35和20.95 +/- 0.01 g DW/l。EPS的产量达到0.87 +/- 0.05（STR）和0.75 +/- 0.05 g/l（烧瓶），IPS的总产量达到2.49 +/- 0.07（STR）和2.40 +/- 0.05 g/l（烧瓶）。STR和摇瓶的GA总产量分别达到367.1 +/- 17.4和297.9 +/- 13.7 mg/l，其相应的生产率为29.6 +/- 0.5和14.3 +/- 0.1 mg/l/天。无论是菌体密度，还是GA和IPS的含量、产量和产率均为G. lucidum。(C)2002年爱思唯尔科技有限公司All rights reserved.
A process for simultaneous efficient production of bioactive ganoderic acid (GA) and Ganoderma polysaccharide by fed-batch fermentation of Ganoderma lucidum was developed. Effects of carbon source and initial sugar concentration oil the production of GA and polysaccharide were studied at first. Sucrose as a carbon source was suitable for the extracellular polysaccharide (EPS) production although the cells could not grow well. Lactose was beneficial for the cell growth and production of GA and intracellular polysaccharide (IPS). When the initial lactose concentration exceeded 35 g/l, the GA accumulation was decreased. The GA production was remarkably improved by pulse feeding of lactose, when its residual concentration was between 10 and 5 g/l. The fed-batch fermentation process in shake flasks could be successfully reproduced in stirred bioreactors (STR) with dissolved oxygen (DO) controlled between 20 and 35% of air saturation during fermentation. The maximum cell density reached 21.89 +/- 0.35 and 20.95 +/- 0.01 g DW/l in STR and flasks, respectively. The production of EPS reached 0.87 +/- 0.05 (STR) and 0.75 +/- 0.05 g/l (flask), and the total production of IPS reached 2.49 +/- 0.07 (STR) and 2.40 +/- 0.05 g/l (flask). The total production of GA reached 367.1 +/- 17.4 and 297.9 +/- 13.7 mg/l for STR and flask, respectively, and their corresponding productivity was 29.6 +/- 0.5 and 14.3 +/- 0.1 mg/l per day. Not only the cell density but also the content, production and productivity of GA and IPS obtained in this work are the highest reported in agitated fermentation of G. lucidum. (C) 2002 Elsevier Science Inc. All rights reserved.