Mechanisms for S-S and N-Cα bond cleavage in peptide ECD and ETD mass spectrometry
Mechanisms for S-S and N-Cα bond cleavage in peptide ECD and ETD mass spectrometry
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DOI：
10.1016/j.cplett.2009.10.062
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发表时间：
2010-01-07
影响因子：
2.8
通讯作者：
Simons, Jack
中科院分区：
文献类型：
--
作者：
Simons, Jack
这封信回顾了在质谱分析中电子捕获和电子转移解离键裂解的机制。主要的问题包括电子最初附着在母离子的哪里，在最初的电子捕获步骤中释放的能量是否是决定哪个键将被切割的关键，电子是否可以从它最初附着的位置迁移到母离子的其他位置，如果是的话，迁移的距离和速率是多少，以及为什么在多肽中，人们发现二硫键和n-c - α键主要被切割。(C) 2009 Elsevier B.V.版权所有
This Letter reviews efforts made to elucidate the mechanism by which electron-capture and electron-transfer dissociation bond cleavages occur in mass spectrometry. The primary issues include where in the parent ion the electron initially attaches, whether the energy released in this initial electron-capture step is key to determining which bonds will cleave, whether the electron can migrate from the site to which it initially attaches to other sites in the parent ion, and, if so, over what distances and at what rates, and why, in polypeptides, one finds disulfide and N-C-alpha bond cleavage primarily. (C) 2009 Elsevier B.V. All rights reserved.