A Rice Lamina Inclination Test—A Micro-quantitative Bioassay for Brassinosteroids
A Rice Lamina Inclination Test—A Micro-quantitative Bioassay for Brassinosteroids
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DOI：
10.1271/bbb1961.48.719
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发表时间：
1984-03
期刊：
影响因子：
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通讯作者：
K. Wada;S. Marumo;H. Abé;T. Morishita;Kenjid . Nakamura;M. Uchiyama;K. Mori
中科院分区：
文献类型：
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作者：
K. Wada;S. Marumo;H. Abé;T. Morishita;Kenjid . Nakamura;M. Uchiyama;K. Mori
以水稻品种Arborio j - 1和Nihonbare为样品，采用简便、特异的水稻叶片倾斜试验，在5 × 10-5 /ig/ml ~ 5 × 10-3μg /ml浓度范围内测定油菜素内酯1及其6-酮类同系物castasterone 2的微量含量。植物激素吲哚-3-乙酸（IAA）在该生物试验中显示出类似的活性。其最低有效浓度为50 /ig/μl，比油菜素内酯高5个数量级。其他植物激素，脱落酸（ABA）和细胞分裂素-动素和a6 -苄基腺嘌呤抑制水稻幼苗的叶片倾斜。细胞分裂素的加入降低了油菜素内酯的促进作用。因此，水稻层倾角试验既可以作为油菜素内酯的微量生物测定方法，也可以作为抗油菜素内酯化合物的检测方法。
A rice lamina inclination test that is simple and specific for brassinosteroids was used as a micro-quantitative bioassay for brassinolide 1 and its 6-keto congener, castasterone 2, in the concentration range of 5 x 10–5 /ig/ml to 5 x 10–3μg/ml, when uniform seedlings of the rice cultivars Arborio J-l and Nihonbare were selected. A phytohormone, indole-3-acetic acid (IAA), showed similar activity in this bioassay. Its lowest effective concentration, however, was 50 /ig/μl, about five orders of magnitude greater than that of brassinolide. Other phytohormones, abscisic acid (ABA) and the cytokinins kinetin and A6-benzyladenine, inhibited the lamina inclination of rice seedlings. The addition of a cytokinin reduced the promoting effect of brassinolide. Thus, the rice lamina inclination test can be used both as a micro-quantitative bioassay for brassinosteroids and as a method for detecting antibrassinolide compouds.