Immunosuppressive effects of an ethyl acetate extract from Urtica dentata Hand on skin allograft rejection
Immunosuppressive effects of an ethyl acetate extract from Urtica dentata Hand on skin allograft rejection
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荨麻乙酸乙酯提取物手部皮肤同种异体移植排斥反应的免疫抑制作用
DOI：
10.1016/j.jep.2009.08.011
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发表时间：
2009-10-29
影响因子：
5.4
通讯作者：
Wang, Xin
中科院分区：
文献类型：
--
作者：
Xiang, Ming;Hou, Wen-Rui;Wang, Xin
目的：探讨齿状荨麻手油皮肤同种异体移植排斥反应小鼠模型中高效液相色谱（HPLC）乙酸乙酯提取物（EAE）的免疫抑制作用。材料与方法：将C57BL/6小鼠同种异体皮肤移植于Balb/c小鼠背部创面，建立异体皮肤移植模型。我们使用FACS研究EAE油树突状细胞（DCs）成熟和CD4(+)CD25(+)T调节细胞（Tregs）分化的影响。我们还研究了dc的脾淋巴细胞增殖和T-bet基因表达。ELISA法检测Th1/Th2细胞因子浓度作为Th1/Th2反应的标志物。结果：EAE能显著延长同种异体皮肤移植体的存活时间，并呈剂量依赖性。通过流式细胞术，我们发现EAE （200mg kg(-1)）治疗可导致dc的所有未成熟陈述，并刺激CD4(+)CD25(+)Tregs的分化。此外，EAE处理小鼠T-bet基因的表达和脾脏淋巴细胞的增殖均得到有效抑制。与模型对照组相比，eae处理的受者明显下调(P
Aim of the study: To investigate the immunosuppressive effects of HPLC qualitied ethyl acetate extract (EAE) from Urtica dentate Hand oil skin allograft rejection ill a murine model.Materials and methods: Allo-skin transplantation model was established by placing skin allograft of C57BL/6 mice in the wound bed which was oil the back of Balb/c Mice. We used FACS to Study the effects of EAE oil dendritic cells (DCs) maturation and CD4(+)CD25(+)T regulatory cells (Tregs) differentiation. We also Studied spleen lymphocyte proliferation and T-bet gene expression in DCs. Concentration of Th1/Th2 cytokines was monitored as markers of Th1/Th2 responses by ELISA.Results: A significant prolongation of skin allografts Survival was observed as a dose-depenclent manner the animals treated with EAE. By FACS, we found that treatment with EAE (200 mg kg(-1)) resulted ill all immature statement of DCs and stimulated the differentiation of CD4(+)CD25(+)Tregs. Additionally, the expression of T-bet gene and the proliferation of spleen lymphocytes were efficiently abated in EAE treated mice. comparing to the model control, EAE-treated recipients showed a significant down-regulation (P